We narrowed to 29,111 results for: ple

1. pT-GFP-rG1

   Plasmid

   #188967

   Purpose

   IPTG inducible GFP with sgRNA

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   GFP
   sgRNA: agtggaaaacaatgcgaccgactagt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT-GFP-rG2

   Plasmid

   #188968

   Purpose

   IPTG inducible GFP with sgRNA

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   GFP
   sgRNA: agtccatgtaatcagcgtctactagt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. L1_NOP1:mS-N7-Ck2

   Plasmid

   #136126

   Purpose

   L1 in position 2, example of weak promoter

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpNOP1:mScarletI-N7_3tNos-35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. FKBP-EGFP-PML peptide

   Plasmid

   #175247

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   PML (PML Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVD2

   Plasmid

   #165857

   Purpose

   universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSWD95

   Plasmid

   #158617

   Purpose

   CckA FRET Sensor

   Depositor

   Seth Childers

   Article

   Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

   Insert

   CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Xylose

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZS160

   Plasmid

   #134238

   Purpose

   Lentiviral plasmid expressing sgRNA against HJURP

   Depositor

   Iain Cheeseman

   Article

   Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

   Insert

   Human HJURP sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRPL28 CaURA3 (pNTI592)

   Plasmid

   #115431

   Purpose

   URA3-marked RPS28

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   KpnI site in intron

   Promoter

   Ashbya gossypii and endogenous

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-6NBC-GFP-PGK-Puro-Barcode9

   Plasmid

   #83686

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode9
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   please view depositor comments below

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVH004

    Plasmid

    #80401

    Purpose

    Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    CusR (cusR )
    AraC (araC )
    Anti-scaffold
    

    Use

    Synthetic Biology

    Tags

    C-terminal LZx domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-myc-mCE

    Plasmid

    #82459

    Purpose

    Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mRNA capping enzyme (Rngtt Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJexpress414_OmpA171_WTnoGirdles

    Plasmid

    #62653

    Purpose

    Wild-type OmpA with aromatic girdle residues replaced by leucine

    Depositor

    Timothy Whitehead

    Article

    Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

    Insert

    OmpA_noGirdles
    

    Use

    Synthetic Biology

    Tags

    6His and FlAsH

    Expression

    Bacterial

    Mutation

    Aromatic girdle residues mutated to Leu (W7, W15,…

    Promoter

    T7

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG

    Plasmid

    #217711

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-6

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG

    Plasmid

    #217739

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL1 (ADGRL1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-844

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-MBP-TEVpCS-ADGRL3 CTFdeltaTA-FLAG

    Plasmid

    #217741

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL3 (ADGRL3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-847

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-MBP-TEVpCS-ADGRE5 CTFdeltaTA-FLAG

    Plasmid

    #217726

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE5 (ADGRE5 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-536

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-MBP-TEVpCS-ADGRB1 CTFdeltaTA-FLAG

    Plasmid

    #217714

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB1 (ADGRB1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-932

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-MBP-TEVpCS-ADGRF5 CTFdeltaTA-FLAG

    Plasmid

    #217731

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF5 (ADGRF5 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-996

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRG5 CTFdeltaTA-FLAG

    Plasmid

    #217736

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG5 (ADGRG5 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-232

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge