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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21,788 results for: his

Showing: 5491 - 5520 of 21788 results

  1. pRD444

    Plasmid
    #168470
    Purpose
    For the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mNeonGreen-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168470-plasmid-map-sequence-id-331923

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  2. pET-30a(+)-Syk-tSH2-FX

    Plasmid
    #111272
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    interdomain A (Phe 119 to His 162) substituted by…
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111272-plasmid-map-sequence-id-215700

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  3. pBad-HPII

    Plasmid
    #105839
    Purpose
    expresses E. coli HPII catalase with N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105839-plasmid-map-sequence-id-205523

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  7. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Use
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  10. pSG66-KcsA-Kv1.3-sfGFP

    Plasmid
    #102457
    Purpose
    Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Insert
    KcsA-Kv1.3
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    102457-plasmid-map-sequence-id-197242

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  15. pRB30

    Plasmid
    #165480
    Purpose
    pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His
    Depositor
    Renato Bruni
    Article
    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2
    Type
    Empty backbone
    Use
    Tags
    TEV-FLAG-10x His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    165480-plasmid-map-sequence-id-326612

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  28. pSG69-KcsA-Kv1.3-deGFP

    Plasmid
    #102458
    Purpose
    Expresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Inserts
    KcsA-Kv1.3
    deGFP
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102458-plasmid-map-sequence-id-197246

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  29. GLRX3

    Plasmid
    #39036
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX3 (GLRX3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    A PCR artifact adds a C-terminal sequence includi…
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5491 - 5520 of 21788 results