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pBabe-HA-Chk2-D311V

Plasmid

#41921

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D311V

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-ALK2 wt

Plasmid

#80870

Purpose

mammalian expression of ALK2 WT

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK2 (ACVR1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
Hchk2 (kin-)-PUNI10

Plasmid

#41908

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Expression

Bacterial

Mutation

kinase dead (D347A)

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pSG5-HA-p300-DY1399

Plasmid

#89095

Purpose

mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300-D1399Y mutant (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

p300-DY1399

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-230-670

Plasmid

#89099

Purpose

mammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 230-670

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1601-1900

Plasmid

#89102

Purpose

mammalian expression of human p300 amino acids 1601-1900 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1601-1900

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-501-999

Plasmid

#89100

Purpose

mammalian expression of human p300 amino acids 501-999 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 501-999

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLEGFP-S727A-STAT3

Plasmid

#71446

Purpose

Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

S727A

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR-p300HAT

Plasmid

#157795

Purpose

Expression of sfGFP-TetR-p300HAT fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR-p300 (EP300 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3A

Plasmid

#137756

Purpose

Expression of LC3A

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3A (MAP1LC3A Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-hLin28B

Plasmid

#26358

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog B (LIN28B Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo sgTREX1

Plasmid

#127645

Purpose

Knock-out of human TREX1 with NeoR

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

TREX1 sgRNA (TREX1 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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CHRM1-Tango

Plasmid

#66248

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CHRM1 (CHRM1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
PHB2 S39D-mCherry

Plasmid

#232787

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 39 to Aspartate

Promoter

CMV

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1000-1600

Plasmid

#89101

Purpose

mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1000-1600

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-2-300

Plasmid

#89098

Purpose

mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 2-300

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CHKA

Plasmid

#218540

Purpose

human CHKA cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1901-2414

Plasmid

#89103

Purpose

mammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1901-2414

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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sg_Brd9_i5-ipUSEPR-TR657

Plasmid

#228922

Purpose

Knockdown of Brd9 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Brd9_i5 (Brd9 Mouse)

Use

Lentiviral

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Brd9_i2-ipUSEPR-TR657

Plasmid

#228921

Purpose

Knockdown of Brd9 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Brd9_i2 (Brd9 Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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