We narrowed to 4,056 results for: lenti crispr plasmid

1. pXPR_502

   Plasmid

   #96923

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX_311-Cas9

   Plasmid

   #96924

   Purpose

   for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

   Depositor

   John Doench, David Root, William Hahn

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   3xFlag and V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDECKO_mCherry

   Plasmid

   #78534

   Purpose

   Backbone to clone single or paired sgRNAs

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA)

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_501

   Plasmid

   #96922

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLH-sgRNA-Sirius-4X(MS2-PP7)

   Plasmid

   #121941

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-4X(MS2-PP7)
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   human U6 promoter

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X

   Plasmid

   #192151

   Purpose

   T cell CRISPR AAV-SB vector

   Depositor

   Sidi Chen

   Article

   Ye et al Nat Biotechnol. 2019 Nov;37(11):1302-131

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDual_dsCas9_Puro_hs_NC_chr1

   Plasmid

   #214688

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr1
   

   Use

   CRISPR and Lentiviral

   Mutation

   Puromycin resistance cassette has silent mutation…

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDual_dsCas9_Puro_hs_TP73

   Plasmid

   #214689

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73

   Depositor

   Kevin Janes

   Insert

   dgRNA_TP73 (TP73 Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   Puromycin resistance cassette has silent mutation…

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

   Plasmid

   #218558

   Purpose

   Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

   Depositor

   Alejandro Chavez

   Insert

   MCP-MLLx3-HSF1
   

   Use

   Lentiviral

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

    Plasmid

    #218559

    Purpose

    Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-P65-HSF1
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

    Plasmid

    #105043

    Purpose

    Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Stk11 (Stk11 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

    Plasmid

    #105025

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

    Plasmid

    #105026

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid

    #132554

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    

    Expression

    Mammalian

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-W

    Plasmid

    #105021

    Purpose

    Lentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Zfp219 (Zfp219 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W

    Plasmid

    #105030

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ndufa2 (Ndufa2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge