We narrowed to 26,940 results for: sta

1. pTrc99S-ssDsbA-TTc-4xDQNAT

   Plasmid

   #128401

   Purpose

   Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin fragment C domain
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTrc99S-ssDsbA-TTlight-4xDQNAT

   Plasmid

   #128402

   Purpose

   Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pS848∙CsR

   Plasmid

   #199240

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EFS-FlpO

   Plasmid

   #191509

   Purpose

   Lentiviral Flp Recombinase driven by the EFS promoter

   Depositor

   Eric Snyder

   Article

   Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

   Insert

   flp recombinase (flp )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/EFS promoter inserted

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR1A DHFR.cHSF1

   Plasmid

   #134737

   Purpose

   Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

   Insert

   DHFR.cHSF1 (HSF1 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   DHFR

   Expression

   Mutation

   Deletion of amino acids 186-202

   Promoter

   None

   Available since

   Oct. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

   Plasmid

   #123360

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Empty LbCpf1 crRNA cloning cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCosSAD1AprR

   Plasmid

   #190786

   Purpose

   An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

   Depositor

   Jan Kormanec

   Article

   Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

   Inserts

   upstream region of the aur1 cluster for auricin
   ampramycin resistance cassette
   downstream region of the aur1 cluster for auricin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNAintron-CHIKV-nsp1-c3xFLAG

   Plasmid

   #215694

   Purpose

   Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp1
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNAintron-CHIKV-nsp2-c3xFLAG

   Plasmid

   #215695

   Purpose

   Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp2
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNAintron-CHIKV-nsp4-c3xFLAG

    Plasmid

    #215697

    Purpose

    Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp4
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. MSP2253

    Plasmid

    #70704

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Ha-Brca1 S1423A/S1524A

    Plasmid

    #32539

    Depositor

    Michael Kastan

    Article

    Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S1423A/S1524A

    Promoter

    CMV/rabbit B-Globin

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

13. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

    Plasmid

    #172226

    Purpose

    Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Piggybac transposon

    Tags

    3XFLAG, DD-domain, GS-linker, and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. DDX5 D248N V5

    Plasmid

    #199577

    Purpose

    Expresses helicase dead DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    D248N

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. UBQLN2_450-624W

    Plasmid

    #176852

    Purpose

    Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

    Depositor

    Carlos Castañeda

    Article

    Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

    Insert

    UBQLN2_450C_W (UBQLN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deletion of residues 1-449 and addition of C-term…

    Promoter

    T7

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PDF1::mCitrine-MBD

    Plasmid

    #115222

    Purpose

    Microtubule visualization

    Depositor

    Jan Traas

    Article

    Armezzani et al Development. 2018 Jun 4;145(11). pii: de

    Insert

    Microtubule binding domain
    

    Use

    Tags

    mCitrine

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. HOXB2 (human) HIS-tag pET

    Plasmid

    #8522

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB2 (HOXB2 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at AA #2; makes protein by Western

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_SNORD27h

    Plasmid

    #73066

    Purpose

    expression clone for human SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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