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pAd GFP HDAC5 S259A/S498A

Plasmid

#32218

Depositor

Reuben Shaw

Publication

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

GFP

Expression

Mammalian

Mutation

S259A/S498A

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSS2 (hS100B)

Plasmid

#26774

Depositor

Gary Shaw

Publication

Smith et al Biochemistry. 1996 Jul 9. 35(27):8805-14

Insert

S100B (S100B Human)

Expression

Bacterial

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-RPS6KA1

Plasmid

#23860

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA1 (RPS6KA1 Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGwf

Plasmid

#22517

Depositor

Christopher Buck

Publication

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Type

Empty backbone

Expression

Mammalian

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAMS366-4xCDRE-GFP-PEST

Plasmid

#138658

Purpose

destabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Publication

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yEGFP3-PEST

Use

Yeast reporter plasmid

Promoter

CYC1

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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tUI

Plasmid

#122661

Purpose

Express tUI protein in E. coli

Depositor

Robert Cohen

Publication

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tUI (UBQLN1, USP5 Human)

Tags

His-tag

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Publication

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Expression

Yeast

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

Plasmid

#106177

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

hSynapsin

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTX171

Plasmid

#89258

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLKO.1-sh-mSlug-4

Plasmid

#40648

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-4 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pPIGA-PHD

Plasmid

#26778

Depositor

Linzhao Cheng

Publication

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PGK-Hyg-p(A) (PGK1 )

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBabe Smad4J Rescue

Plasmid

#19153

Depositor

Joan Massague

Publication

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

SMAD family member 4 (SMAD4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

G to A at bp 1397 T to C at bp 1398

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV.CAG.iGluSnFR3.v857.SGZ

Plasmid

#178334

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Publication

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-CYFIP1.GFP

Plasmid

#109139

Purpose

GFP-tagged CYFIP1 expression construct

Depositor

Josef Kittler

Publication

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CYFIP1 (CYFIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-Hyg-RFPKDEL

Plasmid

#138660

Purpose

Reporter plasmid expressing the ER-located RFP

Depositor

Bertrand Collet

Publication

Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RFP-KDEL

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

Plasmid

#177850

Purpose

Bacterial Expression of AMPK

Depositor

Karsten Melcher

Publication

Yan et al Science. 2021 Jul 23;373(6553):413-419.

Insert

AMPK (a1b2g1)

Tags

MBP

Expression

Bacterial

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMM119

Plasmid

#127213

Purpose

BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

GFP, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-myc-SNAP-GP135

Plasmid

#50376

Purpose

Express myc and SNAP-tagged GP135 in mammmalian cells

Depositor

Reika Watanabe

Publication

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP

Expression

Mammalian

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article