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p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and her4.3

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-GFPnls

Plasmid

#121938

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

PCP-GFPnls

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.771

Plasmid

#108534

Purpose

NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

NahRAM + PSalTTC-YFP

Expression

Bacterial

Mutation

NahR: I25V P72H V118A R248C

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN155(I152L)

Plasmid

#27098

Depositor

Chang-Deng Hu

Article

Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Insert

bJunVN155(I152L) (Jun Rat, and Aequorea Victoria)

Tags

Myc

Expression

Mammalian

Mutation

I152L in Venus(1-154)

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD)

Plasmid

#176191

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

6xHis

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET251-pUC 12xMS2V6 Loxp KANr Loxp

Plasmid

#104392

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.677

Plasmid

#108530

Purpose

AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AraCAM + AraE + PBAD-YFP

Expression

Bacterial

Mutation

AraC: L133F E165L E169G C280L

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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OSCA3.1

Plasmid

#136596

Purpose

Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA3.1 (ERD4 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-loxSTOPlox-LynEGFP

Plasmid

#133922

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S

Plasmid

#149638

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, empty

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pORE303N

Plasmid

#194438

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertion

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

2x35S

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pKH118_keima-YIPF4

Plasmid

#214971

Purpose

Golgiphagy flux reporter keima-YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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yTREX

Plasmid

#177306

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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Tubulin-LSSmGFP

Plasmid

#186535

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAJM.474

Plasmid

#108526

Purpose

LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LuxR + PLuxB-YFP

Expression

Bacterial

Mutation

VanR: T49I A111V P179S

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3

Plasmid

#200635

Purpose

Negative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

iCasp9

Use

Lentiviral

Promoter

pTRE3G

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBZS-YP_001497877

Plasmid

#198366

Purpose

Express YP_001497877 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001497877 (B681L )

Expression

Bacterial

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RRN3/TIF1A

Plasmid

#17661

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RRN3/TIF1A (RRN3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

See depositor comments below

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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