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Showing: 7361 - 7400 of 10228 results

  8. TA1-DuET

    Plasmid
    #213382
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TA1 (TAAR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213382-plasmid-map-sequence-id-430737

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  11. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid
    #101239
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    Fgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101239-plasmid-map-sequence-id-199088

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  13. pAWH-largeT

    Plasmid
    #133895
    Purpose
    Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Insert
    LargeT
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    T7
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    133895-plasmid-map-sequence-id-266967

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  15. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid
    #59313
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59313-plasmid-map-sequence-id-107095

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  16. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Use
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Article
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Use
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

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  27. pET21-Drp1

    Plasmid
    #72927
    Purpose
    Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Drp1 (Dnm1l Mouse)
    Use
    Tags
    6xHis-tag and PreScission Protease site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72927-plasmid-map-sequence-id-137706

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  30. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  31. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid
    #59314
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59314-plasmid-map-sequence-id-107094

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  32. pET-alpha-synuclein-BiFC

    Plasmid
    #87855
    Purpose
    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    alpha-synuclein-VC155
    alpha-synuclein-VN154
    Use
    Tags
    C-terminal fragment of mVenus BiFC protein and N-…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87855-plasmid-map-sequence-id-175017

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  33. pACM4-ACA

    Plasmid
    #49809
    Purpose
    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent site-directed mutagenesis to remove SalI s…
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49809-plasmid-map-sequence-id-74624

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  40. pETM6-FAS

    Plasmid
    #49810
    Purpose
    Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    fatty acyl-ACP thioesterase
    hydroxydecanoyl-ACP dehydratase
    b-ketoacyl-ACP synthase III
    3-ketoacyl-ACP reductase
    enoyl-(acyl carrier protein) reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon-optmized. See supplemental information of …
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49810-plasmid-map-sequence-id-74625

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Showing: 7361 - 7400 of 10228 results