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We narrowed to 9,370 results for: bli

Showing: 7361 - 7400 of 9370 results

  4. SOX2-3'gRNA

    Plasmid
    #215543
    Purpose
    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in
    Depositor
    Kai Wang
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215543-plasmid-map-sequence-id-424976

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  5. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid
    #215544
    Purpose
    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
    Depositor
    Kai Wang
    Insert
    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    Use
    CRISPR
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    215544-plasmid-map-sequence-id-424979

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  7. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

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  8. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

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  9. GOSR2(G144W)-mCherry

    Plasmid
    #209887
    Purpose
    Expresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    GOSR2 (GOSR2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed glycine 144 to tryptophan; this mutation …
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209887-plasmid-map-sequence-id-413669

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  11. pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

    Plasmid
    #192113
    Purpose
    Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Insert
    SARS-CoV-2-NTD-BA.4/5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192113-plasmid-map-sequence-id-374987

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  12. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid
    #164055
    Purpose
    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164055-plasmid-map-sequence-id-327673

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  13. mouse KANK3 Ankyrin Repeats pET151

    Plasmid
    #164056
    Purpose
    Expresses mouse KANK3 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164056-plasmid-map-sequence-id-327670

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  14. mouse KANK4 Ankyrin Repeats pET151

    Plasmid
    #164057
    Purpose
    Expresses mouse KANK4 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164057-plasmid-map-sequence-id-327675

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  16. pCDZ2FE_Champ1_1-812_2PA

    Plasmid
    #105676
    Purpose
    Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P336A, P341A
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105676-plasmid-map-sequence-id-203181

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  17. pT5T_Rev7_1-211_SA112_KVK_YAK

    Plasmid
    #105630
    Purpose
    Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3
    Depositor
    Thanos Halazonetis
    Insert
    Rev7 (Mad2l2 Mouse)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105630-plasmid-map-sequence-id-203820

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  18. MS2_SC2_S_B.1.640

    Plasmid
    #180228
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    B.1.640 (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    180228-plasmid-map-sequence-id-352513

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  19. CD44U-c010

    Plasmid
    #175453
    Purpose
    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175453-plasmid-map-sequence-id-353218

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  20. MS2_SC2_S_C.1.2

    Plasmid
    #177367
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    C1.2 (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 20, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177367-plasmid-map-sequence-id-347828

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  21. MS2_SC2_S_Mu

    Plasmid
    #177366
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Mu SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Mu (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 20, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177366-plasmid-map-sequence-id-347825

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  22. MS2_SC2_S_Kappa

    Plasmid
    #175953
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Kappa SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Kappa (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175953-plasmid-map-sequence-id-343945

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  23. MS2_SC2_S_Eta

    Plasmid
    #175954
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Eta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175954-plasmid-map-sequence-id-343942

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  24. MS2_SC2_S_Gamma

    Plasmid
    #175047
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Gamma (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175047-plasmid-map-sequence-id-343946

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  25. MS2_SC2_S_Theta

    Plasmid
    #175951
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Theta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175951-plasmid-map-sequence-id-343941

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  26. MS2_SC2_S_Zeta

    Plasmid
    #175952
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Zeta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175952-plasmid-map-sequence-id-343947

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  27. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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  28. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  30. pB6-Id1-T2A-tdTomato-FNF-TK

    Plasmid
    #139281
    Purpose
    B6J Id1-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Available Since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    139281-plasmid-map-sequence-id-317908

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  31. pB6-Gfap-T2A-td-sfGFP-FNF-DTA

    Plasmid
    #137044
    Purpose
    B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    T2A-td-sfGFP
    Expression
    Mammalian
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    137044-plasmid-map-sequence-id-274424

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  35. Ad-mMKP3 S159A S197A-Flag

    Plasmid
    #131579
    Purpose
    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells
    Depositor
    Haiyan Xu
    Insert
    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    Use
    Adenoviral
    Tags
    Flag
    Mutation
    changed Serine 159 to Alanine and Serine 197 to A…
    Promoter
    CMV
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131579-plasmid-map-sequence-id-256812

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Showing: 7361 - 7400 of 9370 results