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Lenti-sgFam135b#1/Cre

Plasmid

#193213

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Fam135b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFam135b#1 (Fam135b Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_1745G>U

Plasmid

#173812

Purpose

mir218 binding site2 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT1745 ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_473G>A

Plasmid

#173811

Purpose

mir218 binding site1 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT473 ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_Q853L

Plasmid

#174008

Purpose

Fibronectin receptor binding site point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

FibronR ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mature ISG15 C-term. Domain (79-165, W123A)

Plasmid

#110765

Purpose

Bacterial expression of mutant proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

proISG15 C-term. domain (79-165, W123A) (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Mutation

W123A

Promoter

T7

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-3HA

Plasmid

#108398

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Tags

3HA

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-eGFP

Plasmid

#108397

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-eGFP-3HA

Plasmid

#108396

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Tags

eGFP-3HA

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_p.N312S

Plasmid

#81574

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Mutation

K5R; N312S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_p.D463N

Plasmid

#81460

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Mutation

K5R; D463N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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LMTK2 gRNA (BRDN0001145198)

Plasmid

#76173

Purpose

3rd generation lentiviral gRNA plasmid targeting human LMTK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LMTK2 (LMTK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LMTK2 gRNA (BRDN0001145764)

Plasmid

#76174

Purpose

3rd generation lentiviral gRNA plasmid targeting human LMTK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LMTK2 (LMTK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LMTK2 gRNA (BRDN0001148094)

Plasmid

#76175

Purpose

3rd generation lentiviral gRNA plasmid targeting human LMTK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LMTK2 (LMTK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TKFC gRNA (BRDN0001148963)

Plasmid

#75785

Purpose

3rd generation lentiviral gRNA plasmid targeting human TKFC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TKFC (DAK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TKFC gRNA (BRDN0001149092)

Plasmid

#75786

Purpose

3rd generation lentiviral gRNA plasmid targeting human TKFC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TKFC (DAK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Isl1_2A_Lhx3-BSD

Plasmid

#226282

Purpose

Tetracycline-inducible expression of Isl1 and Lhx3 for direct reprogramming of canine fibroblasts to induced-motor neurons

Depositor

Susannah Sample

Article

Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

Insert

ISL1:T2A:LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Tags

Isl1 and Lhx3 linked via T2A

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2_2A_Sox11-PURO

Plasmid

#226284

Purpose

Tetracycline-inducible expression of Ngn2 and Sox11 for direct reprogramming of canine fibroblasts to induced-motor neurons

Depositor

Susannah Sample

Article

Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

Insert

NGN2:T2A:SOX11 (SOX11, NEUROG2 Human)

Use

Lentiviral

Tags

NGN2 and SOX11 linked via T2A

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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HNF1B_pLX307

Plasmid

#98342

Purpose

Lentiviral expression of HNF1B

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HNF1B (HNF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase INF enhancer vector_ORI_ISG15

Plasmid

#99322

Purpose

Luciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

enhancer; chr1: 947976 -949995 (ISG15 Human)

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

Plasmid

#228902

Purpose

For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

Depositor

Bryan Roth

Article

Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

Has Service

AAV8

Insert

hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)

Use

AAV and Synthetic Biology

Tags

HA and P2A mCitrine

Expression

Mammalian

Mutation

R108K

Promoter

Chicken Beta Actin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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