165,075 results

1. LBR-mCherry

   Plasmid

   #172446

   Purpose

   Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   lamin B receptor (LBR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCh-Mfn2

   Plasmid

   #141156

   Purpose

   Mammalian expression and labeling mitofusin 2

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   Mitofusin 2 (MFN2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMG-RIS

   Plasmid

   #225132

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MSCV-BCR-ABL1-p190-IRES-EGFP

   Plasmid

   #219964

   Purpose

   Express human BCR-ABL1-P190 in mammalian cells

   Depositor

   Mohamed Fareh

   Insert

   BCR-ABL1-P190 (ABL1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-EGFP-RhoA-Q63L

   Plasmid

   #12968

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA constitutively active (RHOA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q63L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAX APP C99

   Plasmid

   #30146

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   APP C99 (APP Human)
   

   Expression

   Mammalian

   Mutation

   C-terminal from BACE site of APP

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. hZMPSTE24-3FLAG_pQCXIP

   Plasmid

   #89759

   Purpose

   Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

   Depositor

   Martin Dorf

   Article

   Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

   Insert

   Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
   

   Use

   Retroviral; Bicistronic expression: puromycin re…

   Tags

   9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

   Expression

   Mammalian

   Mutation

   Y253H in ZMPSTE24 (see depositor comments below)

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hsyn-GRAB_DA2m

   Plasmid

   #140553

   Purpose

   Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. N-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58693

   Purpose

   Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   3xFlag, GyrA Nsplit Intein, and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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