We narrowed to 6,467 results for: poly

1. pPRIME-CMV-LNGFR-FF3

   Plasmid

   #11666

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   LNGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOPINK-TRABID (NZF1, aa 1-33)

   Plasmid

   #65437

   Purpose

   Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   only codes for amino acids 1-33 of TRABID, which …

   Promoter

   T7

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pGBW-m4046799

   Plasmid

   #145577

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. GAL FLAG25QDProCFPp303

   Plasmid

   #15584

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Use

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. GAL FLAG46QDProGFP p306

   Plasmid

   #15832

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 46Q Delta Pro (HTT Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Oct. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-C-SLC35B2

   Plasmid

   #155037

   Purpose

   Transient expression of EGFP-SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GAL 46Q+ProGFPp416

   Plasmid

   #15581

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 46Q + Pro (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. GAL 25Q+ProGFPp416

   Plasmid

   #15580

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q + Pro (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pPRIME-CMV-Neo-FF3

   Plasmid

   #11665

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   Neo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMVTAG-NEMOL329P

    Plasmid

    #11971

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Leucine329 to Proline

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

11. MAP2-CD4

    Plasmid

    #43916

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human MAP2 promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGST1-GGA3-VHS

    Plasmid

    #44420

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    GGA3-VHS (GGA3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Ad-CBR (GFP/cAPC)

    Plasmid

    #16576

    Depositor

    Bert Vogelstein

    Article

    Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

    Insert

    cAPC (APC Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residues 958-2076

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-NEMOD311N

    Plasmid

    #11968

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOD311N (IKBKG Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Asp311 to Asn

    Promoter

    Available since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

15. GAL 72Q+ProGFPp416

    Plasmid

    #15582

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q + Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. ch-Tog

    Plasmid

    #28290

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    ch-TOG (CKAP5 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    K19R, E118G

    Promoter

    Available since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GAL FLAG25Q+ProGFPp303

    Plasmid

    #15572

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q + Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWL502-crRNA

    Plasmid

    #174383

    Purpose

    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    a crRNA targeting the template strand
    

    Use

    CRISPR; Archaeal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PhaR promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046940

    Plasmid

    #149261

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pMH-HT_ygiF-CHAD

    Plasmid

    #127793

    Purpose

    Expresses the CHAD domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF-CHAD (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 201-433)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge