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Showing: 7401 - 7440 of 10106 results

  11. SpyLigase

    Plasmid
    #51722
    Purpose
    Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SpyLigase
    Use
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51722-plasmid-map-sequence-id-251770

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  15. pUBC_HA_L7Ae-VP64

    Plasmid
    #68421
    Purpose
    Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    L7Ae
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    Promoter
    human UbC
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    68421-plasmid-map-sequence-id-124596

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  16. pLentiN 16E6no*

    Plasmid
    #37445
    Purpose
    Lentiviral expression of HPV 16E6no* in mammalian cells
    Depositor
    Karl Munger
    Article
    Spangle et al J Virol. 2012 May 2.
    Insert
    HPV 16E6no* (E6 HPV)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    V42L
    Promoter
    CMV
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only

  24. mpCHECK2

    Plasmid
    #167576
    Purpose
    The mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.
    Depositor
    Ting Ni
    Article
    Chen et al Genome Res. 2021 Apr 19. pii: gr.270256.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    167576-plasmid-map-sequence-id-329146

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  25. pBabe-Kras S17N

    Plasmid
    #58899
    Purpose
    Expresses human Kras with a S17N mutation
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    S17N mutation
    Promoter
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    58899-plasmid-map-sequence-id-158643

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  26. pJB158

    Plasmid
    #202601
    Purpose
    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.
    Depositor
    Athanasios Typas
    Article
    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202601-plasmid-map-sequence-id-412308

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  28. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    63901-plasmid-map-sequence-id-136457

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  30. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid
    #216377
    Purpose
    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)
    Depositor
    William Weis
    Insert
    LDL receptor related protein 6 (LRP6 Human)
    Use
    Baculovirus
    Tags
    9xHis and HA signal sequence-FLAG-3C cleavage sit…
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin (LRP6)/p10 (MESD)
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216377-plasmid-map-sequence-id-433174

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  37. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

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  39. pNCMV 16E6no*

    Plasmid
    #37454
    Purpose
    Expression of HPV 16E6no* in mammalian cells
    Depositor
    Karl Munger
    Article
    Spangle et al J Virol. 2012 May 2.
    Insert
    HPV 16E6no* (E6 HPV)
    Use
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    V42L
    Promoter
    CMV
    Available since
    Sept. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 7401 - 7440 of 10106 results