We narrowed to 11,438 results for: 112

1. pGL4.24-Cd36-enhancer8

   Plasmid

   #138580

   Purpose

   Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17923535_17924934

   Depositor

   Lora Hooper

   Article

   Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

   Insert

   Cd36 Promoter (Cd36 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Minimal promoter

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

   Plasmid

   #140014

   Purpose

   Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y126A, Y197F

   Promoter

   EF1

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJM612 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 in TUPV3

   Plasmid

   #161571

   Purpose

   Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV4-SMAC-HA-eGFP

   Plasmid

   #67490

   Purpose

   Targets HA-eGFP to mitochondrial intermembrane space (IMS)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SMAC (DIABLO Human)
   

   Use

   Tags

   HA-eGFP

   Expression

   Mammalian

   Mutation

   aa1-59

   Promoter

   attenuated CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Venus-Bik-pEGFP-C1

   Plasmid

   #166737

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Bik (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ArpC4_pLib

   Plasmid

   #173681

   Purpose

   ArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   ArpC4 (ARPC4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-G2ZYP2

   Plasmid

   #103110

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SPRR2A-pFastBac I

   Plasmid

   #182460

   Purpose

   Expression His-tagged SPRR2A in insect cells

   Depositor

   Lora Hooper

   Article

   Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

   Insert

   small proline rich protein 2A (SPRR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3 Flag MFF iso5 WT

   Plasmid

   #74378

   Purpose

   Flag tagged human MFF isoform 5

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid

    #101719

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px335-EF-Slc2a3-L

    Plasmid

    #122304

    Purpose

    Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p10xUAS-IVS- Syn21-Voltron-p10

    Plasmid

    #119041

    Purpose

    UAS-driven expresion of Voltron in Drosophila

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184739

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T-1 3xHA-MKK7a1-EE

    Plasmid

    #47578

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Residues 73-420 of RefSeq sequence. Ser271 to Gl…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid

    #114108

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

    Insert

    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUW-tetO-Irf4

    Plasmid

    #139804

    Purpose

    doxycycline-inducible expression of mouse Irf4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf4 (Irf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac-HS-NLRC4Δcard

    Plasmid

    #177276

    Purpose

    Purification of mouse NLRC4 (90-1024) from insect cells

    Depositor

    Hao Wu

    Article

    Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Tags

    HIS-SUMO-PreScission

    Expression

    Insect

    Mutation

    I586T, N761S, M885V, K915R and F972L- please see …

    Promoter

    polyhedrin

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sema4c-AP-His

    Plasmid

    #72030

    Purpose

    Expresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4c (Sema4c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-neo-(HA)3-ε

    Plasmid

    #198531

    Purpose

    Expression of HA-tagged AP-4 ε in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    AP-4 ε (AP4E1 Human)
    

    Use

    Tags

    triple HA tag

    Expression

    Mammalian

    Mutation

    silent substitutions in codons 235, 262, 905 and …

    Promoter

    CMV

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-CH115gp160-QES.c14-754*

    Plasmid

    #123282

    Purpose

    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (CH115) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL4.24-Cd36-enhancer5

    Plasmid

    #138577

    Purpose

    Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17893352_17894696

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 Promoter (Cd36 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Minimal promoter

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ArpC5_pLib

    Plasmid

    #173682

    Purpose

    ArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC5 (ARPC5 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ArpC1_pLib

    Plasmid

    #173678

    Purpose

    ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC1 (ARPC1B Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFUW-tetO-HOXA9

    Plasmid

    #139827

    Purpose

    doxycycline-inducible expression of Human HOXA9 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    HOXA9 (HOXA9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Sema4c-Fc-His

    Plasmid

    #72156

    Purpose

    Expresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4c (Sema4c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. px335-sgRNA-EF(Cas9wt)-Rif1

    Plasmid

    #122306

    Purpose

    Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Rif1 (Rif1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUW-tetO-ID2

    Plasmid

    #139808

    Purpose

    doxycycline-inducible expression of Human ID2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    ID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenp

    Plasmid

    #108481

    Purpose

    inducible expression of vsv-CenpB-1-166-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by CenpB 1-166

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA4/TO-ORF66-2xStrep

    Plasmid

    #130953

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length)

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 Flag MFF iso1 WT

    Plasmid

    #74388

    Purpose

    Flag tagged human MFF isoform 1

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

31. p13xLexAOP2-IVS-Syn21-Voltron-p10

    Plasmid

    #119042

    Purpose

    LexAop2-driven expression of Voltron in Drosophila

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUC19-A0LWB3

    Plasmid

    #103070

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3-HA/Bmal L95E

    Plasmid

    #47344

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L95E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pUC19-K1LM04

    Plasmid

    #103119

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTAMAHISTEV_Ab-PiuA

    Plasmid

    #128957

    Purpose

    expresses Acinetobacter baumannii PiuA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    TonB-dependent siderophore receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3 Flag MFF iso4 WT

    Plasmid

    #74390

    Purpose

    Flag tagged human MFF isoform 4

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

37. PTEX150-bio

    Plasmid

    #47788

    Purpose

    Expresses enzymatically monobiotinylated full-length PTEX150 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PTEX150
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p23-mNeongreen-DDX21 HGV

    Plasmid

    #179230

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p23-mNeongreen-DDX21 DAT

    Plasmid

    #179229

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Mammalian

    Mutation

    G234D, K235A

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA6-E2F4CA-Myc

    Plasmid

    #80428

    Purpose

    Mammalian expression of constitutively active chick E2F4

    Depositor

    José María Frade

    Article

    Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

    Insert

    E2F transcription factor 4 (E2F4 Chicken)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T261E, T263E

    Promoter

    CMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUC19-E6I4Q5

    Plasmid

    #103101

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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42. FLAG-SENP2 C548A

    Plasmid

    #126592

    Purpose

    Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

    Depositor

    Joanna Morris

    Article

    Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

    Insert

    SENP2 (SENP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C548A

    Promoter

    CMV

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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43. pCW57.1 tTA SDHA

    Plasmid

    #177976

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to SDHA sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. px335-EF-Slc2a3-R

    Plasmid

    #122305

    Purpose

    Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PHR-LAT Y6F YFFF-mCitrine (XSB589)

    Plasmid

    #78517

    Purpose

    Lentiviral vector for expressing LAT-Y6F YFFF-mCitrine (0 Grb2 binding sites)

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLKO-CreERT2 sgRipk4 v3

    Plasmid

    #158048

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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47. pEJS663-pMCSG7-AcrIIC4Hpa

    Plasmid

    #113440

    Purpose

    Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEJS664-pMCSG7-AcrIIC5Smu

    Plasmid

    #113441

    Purpose

    Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. ArpC2_pLib

    Plasmid

    #173679

    Purpose

    ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC2 (ARPC2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLenti CRISPR V2 sgShmt2_1

    Plasmid

    #106314

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2 (Shmt1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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