We narrowed to 9,415 results for: Coli
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Plasmid#111273Purposeexpress murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS
Plasmid#61940PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 97-100 (N…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
Plasmid#61941PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 117, stop codon introdu…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PIAL2M-wt
Plasmid#67909PurposeInducible expression of PIAL2 in E.coliDepositorInsertPIAL2 (AT5G41580 Mustard Weed)
UseTagsMBPExpressionBacterialMutationE281-A496PromoterP-lacAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 BD (522-645, C643A)/pET28HMT
Plasmid#111073PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 BD (522-645, C643A)PromoterT7Available SinceAvailabilityAcademic Institutions and Nonprofits only -
pES001 MBP-GSK3β-HA-His
Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
UseTagsHA tag, His6, and MBPExpressionBacterialMutationPromoterTacAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMG046 pETARA-CK1α-His
Plasmid#154074PurposeExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA
Plasmid#193728PurposeCre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell typesDepositorInsertClopHensorN
UseAAVTagsStrep-II (N terminal on insert)ExpressionMammalianMutationPromoterEF1aAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Npl4
Plasmid#117108PurposeProduction of recombinant Npl4 in E.coliDepositorAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-G-GECO1.2-ERES
Plasmid#207144PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signalDepositorInsertMcoln1 (MCOLN1 Human)
UseTagsER-Export Signal (ERES) and G-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-BcPC-PLC
Plasmid#224255PurposeExpress N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coliDepositorUseTagsGST and HRV-3C (LEVLFQGP) siteExpressionBacterialMutationThe gene of Bacillus cereus ATCC 14579 was used f…Promoterlac promoterAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-msfGFP-NPM1
Plasmid#237670PurposeFor expression of msfGFP-NPM1 in EcoliDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHis-BRD4 BD1
Plasmid#196544PurposeExpression of BRD4 Bromodomain (BD1) in E.coliDepositorAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST-DHFR Mm
Plasmid#83073PurposeExpresses the full length DHFR Mm in E.coliDepositorAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Ufd1-His
Plasmid#117107PurposeProduction of recombinant Ufd1-His in E.coliDepositorAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core
Plasmid#233618PurposeExpression of His-tagged helicase core region of DDX3X in E.coliDepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-DCX-TurboID-V5-puro
Plasmid#190742PurposeA lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)DepositorInsertDCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1
Plasmid#204558PurposeExpresses GST-tagged UFBP1 in E.coliDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-LMW_FGF2
Plasmid#157657PurposeExpresses FGF2 in E.coli cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
UseTagsHis tagExpressionBacterialMutationchanged Cystein 211,229 to serine, deleted amino …PromoterAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(MGD) (SB264)
Plasmid#227613PurposeInducible expression of Strep-tagged Opa1(MGD) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-580 (MGD) and aa 938-960PromoterT7Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF019 MBP-β-catenin-His
Plasmid#154070PurposeExpresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEL16
Plasmid#107922PurposeHIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYB11-PTBP1-RRM2CCtoSS
Plasmid#89154Purposerecombinant expression of PTBP1-RRM2 C250S,C251S in e.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
UseTagsIntein and chitin binding domainExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEL10
Plasmid#107916PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEL17
Plasmid#107923PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_G47E
Plasmid#101779PurposeExpresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationG47E mutationPromoterT5Available SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf9b
Plasmid#156485PurposeBacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tagDepositorInsertOrf9b
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEL12
Plasmid#107918Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS-mTagRFP-TUBA1B
Plasmid#62850PurposeDonor vector for gene editing at human TUBA1B locusDepositorInsertmTagRFP-TUBA1B (TUBA1B Human, Synthetic)
UseYeast-e.coli shuttle vectorTagsExpressionMutationPromoterAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUMO CPTP
Plasmid#170735PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
rgef-1p::NmBirA
Plasmid#79971Purposeencodes E.coli biotin-ligase under neuronal promotor for C.elegansDepositorInsertBirA
UseTagsNLS::mycExpressionWormMutationPromoterrgefAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEL15
Plasmid#107921Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMG058 MBP-GS 634-666-His
Plasmid#154077PurposeExpresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEL14
Plasmid#107920PurposeLEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST15.1-hRPN13 (285-386)
Plasmid#61936PurposeExpression of hRPN13 (285-386) in E.coliDepositorInserthRPN13 (285-386) (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationConstruct starts at residue 285, stop codon intro…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Human, Synthetic)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG R106L-CPTP
Plasmid#170744PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG K60A-CPTP
Plasmid#170743PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM12CCtoSS
Plasmid#89155PurposeRecombinant expression of PTBP1-RRM12 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
UseTagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_L58R
Plasmid#101780PurposeExpresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationL58R mutationPromoterT5Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-His-GvpC-Calpain
Plasmid#153295PurposeExpresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertCalpain-sensitive Gas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutation1) Contains an extra glycine after the methionine…PromoterT7Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
delNC C-His Ana GvpC in pET28a
Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
UseTagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911(pL91)
Plasmid#124390PurposeE.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AB (325-557, Δ 368-523)/pET28HMT
Plasmid#111052PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AB (325-557, Δ 368-523)PromoterT7Available SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEL11
Plasmid#107917PurposeamdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAD123_hIrg1_N152K,M154I_pvp008
Plasmid#239118PurposehACOD1 with N152K and M154I mutations in E.coli expression vectorDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationDeleted amino acids 1-3 and 462-end. Mutated N152…PromoterAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pColdI-SBP-DDX3_helicase_core
Plasmid#233622PurposeExpression of SBP-tagged helicase core region of DDX3X in E.coliDepositorInsertSBP-DDX3X helicase core (EIF4A1 Human)
UseTagsHis6-SBPExpressionBacterialMutationPromoterAvailable SinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only