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We narrowed to 14,145 results for: TIM

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Showing: 9251 - 9300 of 14145 results

  1. pZ8-Ptac

    Plasmid
    #74064
    Purpose
    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74064-plasmid-map-sequence-id-138074

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  4. pAAV-UbC-mNeonGreen

    Plasmid
    #124102
    Purpose
    Expression of the fluorescent protein mNeonGreen in mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mNeonGreen
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Codon usage was optimized for Homo Sapiens
    Promoter
    UbC
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124102-plasmid-map-sequence-id-240595

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  5. pCDH-Flag-hS1pr1-GFP-Neo

    Plasmid
    #203708
    Purpose
    Overexpression of FLAG-S1PR1-GFP fusion.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-GFP (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag and GFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203708-plasmid-map-sequence-id-403683

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  6. pHH0103 IQGAP1 WW domain

    Plasmid
    #104279
    Purpose
    Bacterial expression of WW domain from IQGAP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    IQGAP1 WW domain (IQGAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104279-plasmid-map-sequence-id-203685

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  11. TAF1L

    Plasmid
    #39014
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1L (TAF1L Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. R777-E112 Hs.IRS2-nostop

    Plasmid
    #70396
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70396-plasmid-map-sequence-id-125845

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  15. pIA033

    Plasmid
    #200420
    Purpose
    thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Tags
    bsPAmCherry
    Expression
    Bacterial
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200420-plasmid-map-sequence-id-403933

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  16. pIA034

    Plasmid
    #200421
    Purpose
    amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Tags
    bsdronPA
    Expression
    Bacterial
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200421-plasmid-map-sequence-id-405458

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  17. pZ8-Prp

    Plasmid
    #74065
    Purpose
    pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    Propionate inducible promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    prp
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74065-plasmid-map-sequence-id-138071

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  18. pCMV-T7-ABEmax-SpG

    Plasmid
    #195278
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195278-plasmid-map-sequence-id-394868

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  21. pKG402

    Plasmid
    #69760
    Purpose
    Retroviral expression of CENP-T aa 256-561
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 256-561 (CENPT Human)
    Use
    Retroviral
    Tags
    mCherry-TEV-S
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pKG194

    Plasmid
    #69757
    Purpose
    Mammalian Expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and eGFP
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

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  31. pHH0103 APBB3 WW domain

    Plasmid
    #104262
    Purpose
    Bacterial expression of WW domain from APBB3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    APBB3 WW domain (APBB3 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104262-plasmid-map-sequence-id-203722

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  32. pHH0103 APBB2 WW domain

    Plasmid
    #104257
    Purpose
    Bacterial expression of WW domain from APBB2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    APBB2 WW domain (APBB2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104257-plasmid-map-sequence-id-201510

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  41. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

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  42. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available Since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  43. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

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  44. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  50. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

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Showing: 9251 - 9300 of 14145 results