We narrowed to 24,606 results for: Nes

1. pX330A_D10A-1x3

   Plasmid

   #58773

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC-1-CmYLCV-4

   Plasmid

   #197694

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   CmYLCV promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-Z25

   Plasmid

   #120068

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   YFP-LID
   

   Use

   Pcr template

   Tags

   YFP-LID

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

   Plasmid

   #133334

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK 3XFLAG-MKK4-EE-NLS hygro

   Plasmid

   #87779

   Purpose

   Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pMaCTag-P08

   Plasmid

   #120019

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mClover3
   

   Use

   Pcr template

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRANS_263

   Plasmid

   #91127

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nano-lantern(Ca2+)_600/pRSETB

   Plasmid

   #51976

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-2G
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMaCTag-Z03

   Plasmid

   #120046

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   moxCerulean3
   

   Use

   Pcr template

   Tags

   moxCerulean3

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGE-attBCC-FRT-mW (pTL788)

    Plasmid

    #115159

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-CC FRT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX302-PTP-SL-V5 puro

    Plasmid

    #87774

    Purpose

    Expresses V5 tagged human PTP-SL

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    PTP-SL (PTPRR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Khc73-GFP

    Plasmid

    #31746

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Khc73 (Khc-73 Fly)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Metallothionein

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pMA5.1 (mouse integrin alpha 5 probe)

    Plasmid

    #14097

    Depositor

    Richard Hynes

    Article

    Yang et al Development. 1993 Dec . 119(4):1093-105.

    Insert

    Mouse Alpha 5 Integrin (Itga5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. Nano-lantern-Actin/pEGFP-C1

    Plasmid

    #51972

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMaCTag-Z14

    Plasmid

    #120057

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeptune2.5
    

    Use

    Pcr template

    Tags

    mNeptune2.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-H9-mCherry

    Plasmid

    #66532

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H9

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H9

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-Z16

    Plasmid

    #120059

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Dronpa3
    

    Use

    Pcr template

    Tags

    Dronpa3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nano-lantern-peroxisome/pcDNA3

    Plasmid

    #51974

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    SKL

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Nano-lantern(Ca2+)_300/pRSETB

    Plasmid

    #51977

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. CT-Nano-lantern(ATP1)/pRSETB

    Plasmid

    #51990

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    6xHis and chloroplast transit peptid

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only