We narrowed to 10,343 results for: aga

1. pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

   Plasmid

   #162486

   Purpose

   For generating transgenic mice expressing ERK biosensor and RGECO1

   Depositor

   Michiyuki Matsuda

   Article

   Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

   Insert

   ERK biosensor based on FRET, and RGECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pATLIC

   Plasmid

   #110287

   Purpose

   A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

   Depositor

   In-Geol Choi

   Article

   Ko et al Appl Environ Microbiol. 2012 May;78(9):3

   Insert

   yfaL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCNL-N1_vinculin

   Plasmid

   #65691

   Purpose

   Expresses Vinculin-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   vinculin (Vcl Mouse)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

   Plasmid

   #87694

   Purpose

   Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   ERT2-PhoCl-Cre-PhoCl-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 768 pME mApple-CAAX

   Plasmid

   #195961

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mApple-CAAX (membrane)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGF-mSox2-ONL

   Plasmid

   #65719

   Purpose

   mSox2 reporter plasmid (expresses ONL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   murine Sox2 promoter and ONL
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVk7-33G1-BioLHis

   Plasmid

   #58949

   Purpose

   backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   6His tag and biotinylation peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-6011nes

   Plasmid

   #108652

   Purpose

   An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-ERK-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP Gamma 2

   Plasmid

   #36102

   Depositor

   Narasimhan Gautam

   Article

   Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

   Insert

   G protein subunit Gamma 2 (GNG2 Bovine)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pHis2-Arf1 (17-181)-Q71L

    Plasmid

    #184808

    Purpose

    Bacterial expression of Arf1 (aa17-181, Q71L mutation)

    Depositor

    James Hurley

    Article

    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    aa 17-181 only, Q71L

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK083 desmaMCS:minpromEGFP,exorh:mCherry

    Plasmid

    #200016

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK074 p5E exorh:EGFP

    Plasmid

    #195951

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Luciferase shRNA

    Plasmid

    #225343

    Purpose

    shRNAmir backbone for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    Luciferase shRNA (LOC116160065 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mcherry Beta 1

    Plasmid

    #36111

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein beta subunit B1 (GNB1 Bovine)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pCSIIbsr-6013x

    Plasmid

    #108654

    Purpose

    An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    Raichu-EV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK278 (mClover-Hygro)

    Plasmid

    #72794

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Hygro
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1/TO-myc-BirA*-NES

    Plasmid

    #112714

    Purpose

    For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    HIV Rev nuclear export signal (NES)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPR-sgPREX1

    Plasmid

    #86127

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    gRNA targeting PREX1 (PREX1 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-D4 miR30-MORC2 ts1

    Plasmid

    #115886

    Purpose

    MORC2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    MORC2 shRNA (MORC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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