We narrowed to 16,539 results for: puromycin

1. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

   Plasmid

   #213118

   Purpose

   Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   PKAsub-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRhoA2G

   Plasmid

   #40179

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. SETD2(SET)-Cas9

   Plasmid

   #186700

   Purpose

   Plasmid encoding SETD2-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   SETD2(SET)-Cas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SadCas9 KRAB

   Plasmid

   #188504

   Purpose

   Expresses FLAG tagged SadCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV CreERT2 puro

   Plasmid

   #22776

   Purpose

   Retroviral expression of tamoxifen-inducible Cre-ERT2

   Depositor

   Tyler Jacks

   Article

   Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

   Insert

   CreERT2
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. Puro.Cre empty vector

   Plasmid

   #17408

   Purpose

   Lentiviral expression of Cre from the mouse PGK promoter

   Depositor

   Tyler Jacks

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

   Insert

   Cre
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse PGK

   Available Since

   March 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459V2.0-HypaCas9

   Plasmid

   #108294

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-opto-mCasp1

   Plasmid

   #208781

   Purpose

   Inducibly expresses mouse caspase1 fused to light activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mCaspase1 Cry2 (Casp1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry, Cry2

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mScarlet-EFS-Puro

   Plasmid

   #227259

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT GLUT1-FLAG

    Plasmid

    #200094

    Purpose

    WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

    Plasmid

    #119903

    Purpose

    Bxb1 integration plasmid for cuO array with Puro selection cassette

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    cuOx144 array
    

    Use

    Mouse Targeting

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLIX402 PHF2

    Plasmid

    #91700

    Purpose

    inducible lentiviral expression of PHF2

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    PHF2 (PHF2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_TRE_sg30-PDGFR_EF1a_mCherry

    Plasmid

    #186309

    Purpose

    Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg30-PDGFR
    mCherry
    

    Tags

    His, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-CAG-hrGFP

    Plasmid

    #52344

    Purpose

    AAVS1 donor vector for constitutive gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    hrGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid

    #186310

    Purpose

    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg61-PDGFR
    eGFP
    

    Tags

    His, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas12j3 VPR

    Plasmid

    #188517

    Purpose

    Expresses FLAG tagged dCas12j3 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB_TRE_Z18-PDGFR_EF1a_eGFP

    Plasmid

    #186304

    Purpose

    Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z18-PDGFR
    eGFP
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti X1 Puro empty (w610-1)

    Plasmid

    #20953

    Purpose

    3rd gen lentiviral empty control vector, Puro

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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