user-defined upper limit for the number of target sequences returned
Alignment
region of similarity between target and query sequences
E-value
a BLAST statistic representing the significance of an alignment, values close to zero
indicate high sequence similarity with low probability of the similarity occurring by chance
Identities
the number of exact nucleotide matches over the alignment, expressed as a fraction
and a percentage
Query Coverage
the length of the query sequence that matches the target sequence in the
alignment
Bit Score
a BLAST statistic measuring the quality of an alignment, higher values indicate a
more significant match
Span
the length of the alignment, including gaps
About Search by Sequence
Search by Sequence performs a nucleotide-nucleotide BLAST search against Addgene’s plasmid sequence database.
BLAST returns plasmids with similarity to the query sequence.
Results are sorted by E-value, a statistic from BLAST that describes the significance of a match.
Lower values are considered better matches.
FASTA headers and numbers at the beginning of each line will be removed.
The query should only contain DNA characters.
Tips for Success
Enter a distinct sequence that is an important, differentiating feature. For example, the coding region of
a gene, instead of the plasmid origin of replication.
Inspect the percent identity, query coverage, and alignment details to determine if a result match is satisfactory.
Visit the corresponding plasmid webpage to view additional details about a matching plasmid.
If no results are returned:
Try a different isoform or region of the desired sequence.
Choose a different BLAST database. Try the general “All Addgene Plasmids” (default selection),
instead of a specific database, such as “Plant Expression Plasmids”
Try selecting a different BLAST algorithm:
megablast: Designed for comparing sequences within the same, or closely related, species.
Default selection.
blastn: Designed for comparing sequences from different species. May return additional results,
if exact species match is not required.
blastn-short: Optimized for searching with shorter sequences (<= 30 nucleotides)
but can still be effective with slightly larger sequences.
There may not be a match in our database.
You can adjust the Max Results setting on the results page from 25 to 500. If many sequences share the same top E-value,
only a truncated set of equally high-scoring matches will be shown. Set the Max Results to 500 to see more matches.
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Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.
Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
Xenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YOL060C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL060C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YOR092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YOR271C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR271C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YOR307C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR307C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YPL189W. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL189W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YPL244C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL244C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YPL264C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL264C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YPR201W. Contains T7 RNA polymerase binding site, and adds polyA tail to YPR201W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YEL004W. Contains T7 RNA polymerase binding site, and adds polyA tail to YEL004W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YER039C. Contains T7 RNA polymerase binding site, and adds polyA tail to YER039C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YHR032W. Contains T7 RNA polymerase binding site, and adds polyA tail to YHR032W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YDL231C. Contains T7 RNA polymerase binding site, and adds polyA tail to YDL231C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YDR338C. Contains T7 RNA polymerase binding site, and adds polyA tail to YDR338C. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YDR352W. Contains T7 RNA polymerase binding site, and adds polyA tail to YDR352W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
Xenopus oocyte expression vector containing YDR438W. Contains T7 RNA polymerase binding site, and adds polyA tail to YDR438W. Used for generation of mRNA, for injection into xenopus laevis oocytes.