We narrowed to 9,512 results for: try
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Plasmid#194752PurposeLentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS
UseLentiviralTagsHAPromoterCMVAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-BS-AtMIR390a-B/c
Plasmid#199559PurposeGATEWAY-compatible entry vectorDepositorInsertBasal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
UseGateway-compatible entry vectorAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-IGFBP2-Clover
Plasmid#191007PurposeGateway entry vector with IGFBP2 tagged with CloverDepositorInsertInsulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
UseGateway entry vectorTagsCloverAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(304-393)
Plasmid#200313Purposebacterial expression of p53 TET-CTDDepositorAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(363-393)
Plasmid#200314Purposebacterial expression of P53 CTDDepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT BirA*-3xFLAG-CSE1L
Plasmid#192312PurposeGateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-CSE1LDepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-In18runon-PTPN22
Plasmid#195377PurposeA human PTPN22 mutant (intron 18-runon) in Gateway pDONR221DepositorAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-In18runon-PTPN22
Plasmid#195379PurposeMammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tagDepositorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Ex1-17-PTPN22
Plasmid#195375PurposeMammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
TagsFLAGExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17Available SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyPromoterPRAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM/UBM-mut
Plasmid#187153PurposeEntry cloneDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUG-T2A-H93R
Plasmid#140739PurposeLentiviral vector to overexpress the autism-associated PTEN mutation H93R and GFP as two separate proteinsDepositorInsertGFP-T2A-PTENH93R (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationH93RPromoterhAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenN276S
Plasmid#140737PurposeLentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion proteinDepositorInsertPTEN – phosphatase and tensin homolog (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationN276SPromoterhUbiCAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenW274L
Plasmid#140736PurposeLentiviral vector to overexpress the autism-associated PTEN mutation W274L as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationW274LPromoterhUbiCAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenD252G
Plasmid#140735PurposeLentiviral vector to overexpress the autism-associated PTEN mutation D252G as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationD252GPromoterhUbiCAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenH93R
Plasmid#140733PurposeLentiviral vector to overexpress the autism-associated PTEN mutation H93R as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationH93RPromoterhUbiCAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)
Plasmid#133791PurposeU6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018DepositorInsertSt1Cas9 sgRNA entry vector
ExpressionMammalianMutationV1 sgRNA architecture from Carter et al. biorxiv …PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)
Plasmid#133792PurposeU6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015DepositorInsertSt3Cas9 sgRNA entry vector
ExpressionMammalianMutationsgRNA architecture from Muller et al. Molecular T…PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS22
Plasmid#129710PurposeExpresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusionDepositorInsertEscherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
Tags10-Histidine tag cleavable with PreScission Prote…ExpressionBacterialMutationRssB E134G:E135S:E136G:E137SPromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only