We narrowed to 14,583 results for: pET

1. PET-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207382

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PET-Blackjack SpCas9-NLS-6xHis

   Plasmid

   #207375

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Blackjack SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-His8-aCat(660-865)

   Plasmid

   #203335

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-865 only

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-His8-aCat(660-885)

   Plasmid

   #203334

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-885 only

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-28a(+)-6xHis-TEV-ORF9c

   Plasmid

   #201023

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   open reading frame 9c
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a- phage MazG-like/Atd1

   Plasmid

   #195244

   Purpose

   Expersses phage MazG-like/Atd1

   Depositor

   Douglas Feldman

   Article

   Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

   Insert

   phage MazG
   

   Use

   Tags

   Flag and TwinStrep

   Expression

   Bacterial

   Mutation

   Catalytic site mutant

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-20b_HtrA2(delta(133))_S306A_6His

   Plasmid

   #197975

   Purpose

   E. coli expression of HtrA2(delta133)-6His

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   delta(1-133), S306A

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM-60-NcQDE2 _506-938-A602E_M

   Plasmid

   #146964

   Purpose

   Bacterial Expression of NcQde-2_506-938-A602E

   Depositor

   Elisa Izaurralde

   Article

   Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

   Insert

   NcQde-2_506-938-A602E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM-60-NcQDE2 _506-938-H609W_M

    Plasmid

    #146965

    Purpose

    Bacterial Expression of NcQde-2_506-938-H609W

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-H609W
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM-60-NcQDE2 _506-938-K634A_M

    Plasmid

    #146966

    Purpose

    Bacterial Expression of NcQde-2_506-938-K634A

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-K634A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM-60-NcQDE2 _506-938-R895A_M

    Plasmid

    #146967

    Purpose

    Bacterial Expression of NcQde-2_506-938-R895A

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-R895A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM-60-NcQDE2 _506-938-Y595L_M

    Plasmid

    #146968

    Purpose

    Bacterial Expression of NcQde-2_506-938-Y595L

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-Y595L
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM-60-HsAGO2-448-859-optim_K

    Plasmid

    #146729

    Purpose

    Bacterial Expression of HsAGO2_448-859-optim

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO2_448-859-optim (AGO2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM-60-NusA-DmEDC3_331-400-ADA_H

    Plasmid

    #146447

    Purpose

    Bacterial Expression of DmEDC3_331-400-ADA

    Depositor

    Elisa Izaurralde

    Insert

    DmEDC3_331-400-ADA (Edc3 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-28a(+)-His(6)-TEV-AtPCO5(wt)

    Plasmid

    #191803

    Purpose

    Full length wt AtPCO5 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO5
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM-41P-HsXRN1_1174-1649-Strep_AH

    Plasmid

    #148901

    Purpose

    Bacterial Expression of HsXRN1_1174-1649

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsXRN1_1174-1649 (XRN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-PLS-KP3-C93A-C96A_rpoA-U

    Plasmid

    #190962

    Purpose

    Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-PLS-KP6-C93A-C96A_rpoA-C

    Plasmid

    #190970

    Purpose

    Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-PLS-KP2-C93A-C96A_rpoA-U

    Plasmid

    #190958

    Purpose

    Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge