We narrowed to 5,300 results for: pcDNA3.1+

1. pcDNA-Stra6L-6His

   Plasmid

   #174215

   Purpose

   Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

   Depositor

   Adrian Press

   Article

   Stafast et al ACS Appl. Polym. Mater. 2022

   Insert

   Stra6L (Stra6l Mouse)
   

   Use

   Tags

   6-His

   Expression

   Mammalian

   Mutation

   non

   Promoter

   CMV

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. WNK2 K207A

   Plasmid

   #24570

   Depositor

   Joseph Costello

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

   Insert

   WNK2` (WNK2 Human)
   

   Use

   Tags

   His6 and V5

   Expression

   Mammalian

   Mutation

   K207A

   Promoter

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pGAL4-DBD-MCS-long

   Plasmid

   #145246

   Purpose

   Backbone vector expressing GAL4 DNA-binding domain followed by MCS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-ZnF/HIT1

   Plasmid

   #15332

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

   Insert

   zinc finger, HIT domain containing 1 (ZNHIT1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

6. POLArIS_NbVim-cp10GFP

   Plasmid

   #171020

   Purpose

   Expresses NbVim-cp10GFP#2 in mammalian cells

   Depositor

   Sumio Terada

   Article

   Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

   Insert

   anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
   

   Use

   Tags

   cp10GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. YFP Rab4

   Plasmid

   #99018

   Purpose

   Localization and visualization of trafficking protein Rab4

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   Rab4 (Rab4 Fly, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcPPK1

   Plasmid

   #108850

   Purpose

   Expresses E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

   Insert

   Polyphosphate Kinase 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP Rab4DN

   Plasmid

   #99019

   Purpose

   Localization and visualization of the dominant negative form of Rab4

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   Rab4 DN (Rab4 Fly, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   S22N Dominant negative

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Flag1-3HABeta2-mEos2

    Plasmid

    #114187

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and mEOS2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

    Plasmid

    #188992

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    K109R, P245A, G328S, G381V, G429D

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 RL3Sc

    Plasmid

    #109365

    Purpose

    Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-ERK2 L198A L232A

    Plasmid

    #8978

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A, L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-HCMVgL-FLAG (Merlin)

    Plasmid

    #136450

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Use

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 PBD wt (Nigg AH12)

    Plasmid

    #41162

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD (PLK1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603)

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pER-eZinCh-2

    Plasmid

    #70009

    Purpose

    expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    ER-eZinCh-2
    

    Use

    Tags

    KDEL sequence and PPI signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dr-EGFR

    Plasmid

    #184004

    Purpose

    Dr-EGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

    Insert

    Dr-EGFR (EGFR Human)
    

    Use

    Tags

    Deinococcus radiodurans bacterial phytochrome

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmyc-GFP-TNRC6A-NES-mut

    Plasmid

    #42001

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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