We narrowed to 10,449 results for: T7
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Plasmid#62939PurposeExpression of Cre-6xHis in bacterial of cellsDepositorInsertCre recombinase
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSH 1-178
Plasmid#160050PurposeExpresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.DepositorInsertAMSH residues 1-178 (STAMBP Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-178PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NES
Plasmid#175008PurposeCytosol-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagā¦ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-37
Plasmid#214346PurposeProtein overexpression in bacteriaDepositorInsertIL37 (IL37 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Flag-Ube2v1
Plasmid#110069PurposeExpress Halo-Tev-Flag-Ube2v1 (human) in mammalian cellsDepositorInsertHalo-Tev-Flag-Ube2v1 (UBE2V1 Human)
UseTagsHalo-TEV-FlagExpressionMammalianMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagā¦ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308A/S473A
Plasmid#49191PurposecDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationT308A; S473APromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
MTNR1A-DuET
Plasmid#213348PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMTNR1A (MTNR1A Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorInsertEB1-GFP-6xHis (Mapre1 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorInsertSARS-COV-2 Nsp12 (ORF1ab Synthetic)
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
UseTagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139ā¦PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev(T/A)
Plasmid#118475PurposeNegative-control mutant for RAB-EKARev biosensor.DepositorInsertRAB-EKARev(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) tā¦ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequencā¦PromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR(T/A)
Plasmid#118463PurposeNegative-control mutant for ExRai-AktAR biosensor.DepositorInsertExRai-AktAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) tā¦ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequencā¦PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialMutationPromoterT7Available sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagā¦ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-E192C
Plasmid#180233PurposeExpresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domains (residueā¦PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to serā¦PromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_human-pro-IL-18
Plasmid#214337PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
UseTags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-SARS-CoV-2-Nucleocapsid
Plasmid#204403PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tagDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 ā¦PromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-HIS-TEV-GST-PP-RPN13
Plasmid#73741PurposeExpression of hRPN13 in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTags6xHIS and GSTExpressionBacterialMutationPromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mTET1
Plasmid#81052Purposebacterial expression of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nrxn1beta AS4(-)-Fc
Plasmid#59313PurposeExpression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]DepositorInsertNrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
UseTagsFc (from human IgG)ExpressionMammalianMutationPromoterCMV, T7Available sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHis2-Arf1 (17-181)-Q71L
Plasmid#184808PurposeBacterial expression of Arf1 (aa17-181, Q71L mutation)DepositorInsertArf1 (ARF1 Human)
UseTagsHis6ExpressionBacterialMutationaa 17-181 only, Q71LPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DrFLINC-AKAR-Kras
Plasmid#182334PurposeGenetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.DepositorInsertDrFLINC-AKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagā¦ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsEDC4_S
Plasmid#147455PurposeMammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.DepositorInsertHsEDC4 (EDC4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dead Streptavidin-SpyTag
Plasmid#59548PurposepET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramersDepositorInsertDead Streptavidin-SpyTag
UseTagsSpyTagExpressionBacterialMutationThe construct lacks final K of SpyTagPromoterT7Available sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-mCherry-6xHis
Plasmid#130984PurposeBacterial expression of mouse EB1-mCherry-6xHis for protein purification.DepositorInsertEB1-mCherry-6xHis (Mapre1 Mouse)
UseTagsmCherry-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
RXFP1-DuET
Plasmid#213373PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertRXFP1 (RXFP1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2-NES
Plasmid#175011PurposeCytosol-targeted AktAR2.DepositorInsertAktAR-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagā¦ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-Drp1
Plasmid#72927PurposeBacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage siteDepositorInsertDrp1 (Dnm1l Mouse)
UseTags6xHis-tag and PreScission Protease siteExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBacT7-S1T3D
Plasmid#33282DepositorInsertS1 (S1 Orthoreovirus T3D)
UseTagsExpressionMammalianMutationPromoterT7Available sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAWH-largeT
Plasmid#133895PurposePositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pBDepositorInsertLargeT
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
hDAGLb-V5
Plasmid#87677PurposeExpresses human DAGLb protein with intracellular V5 tag located on the C-terminalDepositorInsertHuman Diacylglycerol Lipase Beta (DAGLB Human)
UseTagsV5 tagExpressionMammalianMutationPromoterT7Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/L28F
Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a prolineā¦PromoterT7Available sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
TA1-DuET
Plasmid#213382PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertTA1 (TAAR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21 E2F1
Plasmid#90313PurposeExpresses human E2F1 in bacteriaDepositorInsertE2F1 (E2F1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nrxn1beta AS4(+)-Fc
Plasmid#59314PurposeExpression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]DepositorInsertNrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
UseTagsFc (from human IgG)ExpressionMammalianMutationPromoterCMV, T7Available sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only