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Showing: 7481 - 7520 of 10228 results

  5. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

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  6. pET21-10XHis-GST-HRV-Her2-dL5-Her2

    Plasmid
    #85624
    Purpose
    Bacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)
    Depositor
    Marcel Bruchez
    Insert
    10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Schistosoma japonicum, Synthetic)
    Use
    Tags
    10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    85624-plasmid-map-sequence-id-287943

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  12. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid
    #101719
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101719-plasmid-map-sequence-id-199092

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  17. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    James Naismith, Huanting Liu
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Use
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  19. p148

    Plasmid
    #62616
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62616-plasmid-map-sequence-id-102932

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  21. pDC183

    Plasmid
    #106415
    Purpose
    Co-expresses trans SNARE complex with pQZ210 in E. coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
    SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fragment 141-204, Codon optimization and Fragment…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106415-plasmid-map-sequence-id-204169

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  25. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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  40. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

    Plasmid
    #117911
    Purpose
    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM
    Depositor
    Kai Johnsson
    Article
    Yu et al Science. 2018 Sep 14;361(6407):1122-1126
    Insert
    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    R44A & H45Q on eDHFR
    Promoter
    T7
    Available since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    117911-plasmid-map-sequence-id-226235

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Showing: 7481 - 7520 of 10228 results