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pD649-HAsp-IL18R1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156749

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IL18R1 (IL18R1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD84-Fc(DAPA)-AviTag-6xHis

Plasmid

#156616

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD84 (CD84 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IL1R2-COMP5AP-AviTag-9xHis

Plasmid

#157333

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IL1R2 (IL1R2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VSTM5-Fc(DAPA)-AviTag-6xHis

Plasmid

#156494

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSTM5 (C11orf90 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-737pUC19-tNGFR-P2A-CD18

Plasmid

#186113

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD18 (ITGB2 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-456 tNGFR-P2A-ITGB1

Plasmid

#186083

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

ITGB1 (ITGB1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-MFSD9_STOP

Plasmid

#161060

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD9 (MFSD9 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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sgEML4

Plasmid

#85568

Purpose

Expresses sgRNA targeting human EML4 between exon 13 and 14

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

echinoderm microtubule associated protein like 4 (EML4 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
CL20_mEGFP_EML4_ALK

Plasmid

#205809

Purpose

Express mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EML4_ALK (EML4, ALK Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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DRH031_ AAV-SIO-nEF-WT-THR

Plasmid

#225090

Purpose

Cre-dependent expression of wild-type thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

DN-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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DRH032_ AAV-SIO-nEF-DN-THR

Plasmid

#225091

Purpose

Cre-dependent expression of dominant-negative thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

WT-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP4K4

Plasmid

#23486

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K4 (MAP4K4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_MAX_WT_V5

Plasmid

#82944

Purpose

Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1a.PRC2

Plasmid

#234581

Purpose

Expression of EZH2, MBP-SUZ12, EED, RBBP4

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Insect

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE3 (minus strand)

Plasmid

#91845

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0622)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (minus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcrDNA3.1-Chimera

Plasmid

#100891

Purpose

Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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DRH015_ AAV-hSyn-WT-THR

Plasmid

#225088

Purpose

Expression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

WT-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKACA

Plasmid

#23495

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACA (PRKACA Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMY.PRC2

Plasmid

#234582

Purpose

Expression of ProtA-EZH2, SUZ12, EED and RBBP4

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Yeast

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2-IRES2-EBF1

Plasmid

#96966

Purpose

Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)

Tags

FLAG (on EBF1)

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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