We narrowed to 83,558 results for: TRI

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UBQ10:sXVE:ER-GFP1–10

Plasmid

#125664

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRATIO2251

Plasmid

#141305

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET3a-AqAdk

Plasmid

#18089

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad Aquifex aeolicus)

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGL2 enhancer- F2 LUC

Plasmid

#22428

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F2 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Available Since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag-Sesn3

Plasmid

#61869

Purpose

Transient overexpression of Flag-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
GgVcl 1-1066 FRET tail probe 2

Plasmid

#46282

Depositor

Susan Craig

Article

Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-9

Insert

Vcl 1-1066 + mCitrine (VCL Chicken)

Tags

ECFP

Expression

Mammalian

Mutation

mCitrine (and linker) inserted after aa883

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pET3a-AqAdk_Y52C

Plasmid

#18090

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad A. aeolicus)

Expression

Bacterial

Mutation

Tyr 52 changed to Cys

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
PX458_ZNF331_1

Plasmid

#135739

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_2

Plasmid

#135740

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-AMV-δ1L9'S-nAchR

Plasmid

#20687

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1-mCFP

Plasmid

#105142

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mCFP

Tags

81nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mCFP

Plasmid

#105139

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mCFP

Tags

41nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat

Plasmid

#25029

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

HPat (Patr-1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pmECFPC1/GgVcl 1-1066 (CFP at N term)

Plasmid

#46281

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

Vcl 1-1066 (VCL Chicken)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-YC

Plasmid

#107072

Purpose

Host vector to construct a YPer or CyPet fusion protein

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

YPet-CyPet fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3_ctrl_CP-candidate_luc+

Plasmid

#86392

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pH6Z-101

Plasmid

#85226

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTG005

Plasmid

#71386

Purpose

localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

RMP1 (RMP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

ACT1

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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