We narrowed to 87,502 results for: lias

1. pCS2-mRuby-Rab8a

   Plasmid

   #194337

   Purpose

   Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Rab8 (RAB8A Canis lupus)
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDC123SB-AtTAS1c-B/c

   Plasmid

   #51772

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtTAS1c-B/c
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-MtAcKRS (human-opti)

   Plasmid

   #164195

   Purpose

   To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtAcKRS-478(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D76G; L325M; L329I; Y330F; L333A; C372F

   Promoter

   CMV

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-Regnase-1 WT

   Plasmid

   #222653

   Purpose

   Mammalian expression vector to express mouse Regnase-1 WT

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG MCP-VP64

   Plasmid

   #92357

   Purpose

   CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   MCP-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+PhyBΔCreC

   Plasmid

   #131781

   Purpose

   Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLmMBP-2

   Plasmid

   #72344

   Purpose

   Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV.PGK.hLHX6

   Plasmid

   #161828

   Purpose

   Expresses human LHX6(LIM Homeobox 6) in mammalian cells

   Depositor

   Daniella Ottosson

   Article

   Giacomoni et al Cells. 2020 Nov 10;9(11). pii: cells9112

   Insert

   human LHX6 (LHX6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   AA 17-23 deletion

   Promoter

   PGK

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-NLS-gs10-cTRb413

   Plasmid

   #188261

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cTRb413 (TXNRD2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKMD111e - intein-Cas9(S219-G521R)

    Plasmid

    #64192

    Purpose

    Expresses intein-Cas9(S219-G521R) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-M3(T3D)471-721

    Plasmid

    #56657

    Purpose

    GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing

    Depositor

    Max Nibert

    Article

    Broering et al J Virol. 2005 May;79(10):6194-206.

    Insert

    M3(T3D)421-721
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    insert encodes aa 471-721 + native stop codon of …

    Promoter

    CMV-IE

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLin28A-myc-T2A-tagRFP

    Plasmid

    #140267

    Purpose

    Expresses LIN28A in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

    Insert

    LIN28A (LIN28A Human)
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-Med29 pBacPAK8

    Plasmid

    #15369

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    Med29 (MED29 Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. GFP-PKI nls

    Plasmid

    #118301

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI nls (aa 2-36)
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    inserting 2xnls

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-AtTAS1c-B/c

    Plasmid

    #51774

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtTAS1c-B/c
    

    Use

    Gateway-compatible entry clone

    Tags

    Expression

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. CLC-EGFP

    Plasmid

    #193566

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF005-sgRNA-shuffle-in

    Plasmid

    #104441

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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