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pBSKrnaSeph

Plasmid

#67506

Purpose

Express the tRNALys scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pAG26

Plasmid

#35127

Depositor

John McCusker

Article

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

hph

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pLdCN2

Plasmid

#125186

Purpose

Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-Voltron

Plasmid

#119037

Purpose

Pan neuronal expression of Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Zebrafish expression

Promoter

elavl3

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p246

Plasmid

#186183

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 246

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p226

Plasmid

#186181

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 226

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p266

Plasmid

#186185

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 266

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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LRT2B-AdGO2

Plasmid

#136911

Purpose

Lentivirus expressing AdGO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgAdGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-PQRv3-RFP

Plasmid

#73951

Purpose

Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

Depositor

Brian Chen

Article

Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

Inserts

sfGFP (superfolder GFP)
RFP

Tags

A residual PQR peptide wii be left at the C-termi…

Expression

Mammalian

Promoter

Chicken beta actin and Chicken beta actin (shared…

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

Plasmid

#32737

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
GFP-pcDNA3-PKCgamma-cys1Acys1B

Plasmid

#21190

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-cys1Acys1B (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pDA027

Plasmid

#131385

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A, D702Y

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfGFPBb

Plasmid

#118231

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAO139 (Apr LEU2 mrcl-AQ)

Plasmid

#41924

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Mutation

MRC1AQ

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPE4max-Blasticidin

Plasmid

#194905

Purpose

Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

Insert

BSD

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKSJ331

Plasmid

#103063

Purpose

Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

Depositor

Alan Finkelstein, Karen Jakes

Article

Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

Insert

Colicin E1 and E1 immunity protein

Expression

Bacterial

Promoter

colicin promotor from ColE1

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX33

Plasmid

#89632

Purpose

Allelic change mutagenesis

Depositor

Greg Cook

Article

Gebhard et al Microbiology. 2006 Nov;152(Pt 11):3453-6

Insert

xylE

Use

Allelic change mutagenesis

Promoter

Pmyco

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-CFP no-pA

Plasmid

#80827

Purpose

P2A followed by CFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-CFP no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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