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Plasmid#44526DepositorInsertHHT2
UseTagsExpressionYeastMutationhht2 deletion 4-15PromoterAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XMS2 Lentiviral Vector
Plasmid#174198PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviralTags24X MS2 hairpins, Blasticidin, and T2AExpressionMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-Hs4E-T_AF
Plasmid#148687PurposeMammalian Expression of Hs4E-TDepositorInsertHs4E-T (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-Hs4EHP_AE
Plasmid#148588PurposeMammalian Expression of Hs4EHPDepositorInsertHs4EHP (EIF4E2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (334-449)
Plasmid#87859PurposeFluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationfragment from aa 334 to 449PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorInsertGα11-LgB97 (GNA11 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBItetDT480GFP
Plasmid#96905Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15DepositorInsertEGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalTagsExpressionMammalianMutationPromotertetracycline responsive and bidirectionalAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBItetDMPKSGFP
Plasmid#96903Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalTagsExpressionMammalianMutationPromotertetracycline responsive and bidirectionalAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XPP7 Lentiviral Vector
Plasmid#174199PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviral; VectorTags24X PP7 hairpins, Blasticidin, and T2AExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F1shRNA human
Plasmid#66883PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F1DepositorInsertE2F transcription factor 1 (E2F1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F2shRNA human
Plasmid#66884PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F2DepositorInsertE2F transcription factor 2 (E2F2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBItetDT240GFP
Plasmid#96904Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalTagsExpressionMammalianMutationPromotertetracycline responsive and bidirectionalAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-F-API5
Plasmid#157662PurposeExpresses API5 in mammalian cellDepositorInsertApoptosis inhibitor 5 (API5 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/RNAi gamma-tubulin shRNA mouse
Plasmid#70666PurposeReduced the expression of gamma-tubulin in murine cellsDepositorInsertUseU6 promoterTagsExpressionMammalianMutationPromoterU6Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F3shRNA human
Plasmid#66885PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F3DepositorInsertE2F transcription factor 3 (E2F3 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shBirc5
Plasmid#160949PurposeBirc5 shRNA in pMKO.1 retroviral vectorDepositorInsertBirc5 shRNA (Birc5 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (1-333)
Plasmid#87858PurposeFluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationFragment from aa 1 to 333PromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsGIGYF2_AE
Plasmid#148550PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-API5
Plasmid#157655PurposeExpresses API5 in E.coli cellDepositorInsertApoptosis inhibitor 5 (API5 Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_AE
Plasmid#148577PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
API5-CRISPR
Plasmid#157664PurposeExpresses Cas9 and sgRNA targeting API5DepositorInsertApoptosis inhibitor 5 (API5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
D-raf R217L
Plasmid#39762DepositorInsertd-raf (Raf Fly)
UseTagsExpressionInsectMutationR217LPromoterAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-54-GB1_AE
Plasmid#148558PurposeBacterial Expression of HsGIGYF2_35-54DepositorInsertHsGIGYF2_35-54 (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-72-GB1_AE
Plasmid#148559PurposeBacterial Expression of HsGIGYF2_35-72DepositorInsertHsGIGYF2_35-72 (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-105-GB1_AE
Plasmid#148560PurposeBacterial Expression of HsGIGYF2_35-105DepositorInsertHsGIGYF2_35-105 (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-Hs4EHP_1-234_AE
Plasmid#148561PurposeBacterial Expression of Hs4EHP_1-234DepositorInsertHs4EHP_1-234 (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-Hs4EHP_1-234-I85AM101A_AE
Plasmid#148562PurposeBacterial Expression of Hs4EHP_1-234-I85AM101ADepositorInsertHs4EHP_1-234-I85AM101A (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-Hs4EHP_1-234-W95A_AE
Plasmid#148563PurposeBacterial Expression of Hs4EHP_1-234-W95ADepositorInsertHs4EHP_1-234-W95A (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE
Plasmid#148565PurposeBacterial Expression of Hs4EHP_52-234opt-R103LE149LDepositorInsertHs4EHP_52-234opt-R103LE149L (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-Hs4EHP_52-234opt_AE
Plasmid#148566PurposeBacterial Expression of Hs4EHP_52-234optDepositorInsertHs4EHP_52-234opt (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only