We narrowed to 2,360 results for: Efs

1. plex_307-ccdB-g418

   Plasmid

   #201141

   Purpose

   pLEX_307 plasmid with g418 marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICPIS-EF1

   Plasmid

   #73355

   Purpose

   AdenoX shuttle vector with EF-1 promoter

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   EF1

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

   Plasmid

   #216253

   Purpose

   FRET-based calcium sensor located in the ER lumen, Tet-inducible

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   ER-targeted D4 second generation cameleon (FRET calcium sensor)
   

   Use

   Lentiviral

   Tags

   REAch and mTurquoise

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-AsCpf1-Blast

   Plasmid

   #84750

   Purpose

   Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   AsCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF5B-FRT-AP-Sstr3-GFP-DEST

   Plasmid

   #49098

   Purpose

   Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Sstr3 (Sstr3 Mouse)
   

   Use

   Tags

   GFP and acceptor peptide (AP)

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-NRTH

   Plasmid

   #198541

   Purpose

   Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRTH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES_EGFP_GA_KCNE1

   Plasmid

   #173160

   Purpose

   For mammalian expression of KCNE1 and creating variants

   Depositor

   Al George

   Article

   Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

   Insert

   KCNE1 (KCNE1 Human)
   

   Use

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. evoCas9

   Plasmid

   #138558

   Purpose

   Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   evoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-syn-IRES-hrGFP

    Plasmid

    #107549

    Purpose

    high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

11. VQR variant

    Plasmid

    #138560

    Purpose

    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-2TK-WW-CR-CT

    Plasmid

    #18069

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pEXP5-NT-CuSeCat

    Plasmid

    #42568

    Depositor

    Ivan Korendovych

    Article

    Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

    Insert

    mutated calmodulin gene
    

    Use

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Valin…

    Promoter

    T7

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-FLEX-T-RG

    Plasmid

    #102368

    Purpose

    Express cre dependent TVA and SADB19 RG (biscistronic) as an AAV

    Depositor

    Troy Margrie

    Article

    Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

    Insert

    TVA E2A-RG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mycUb-25aa

    Plasmid

    #20256

    Depositor

    Nico Dantuma

    Article

    Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

    Insert

    Ubiquitin-25 amino acid extension (RBX1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pNDGG010

    Plasmid

    #231315

    Purpose

    Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNDGG009

    Plasmid

    #231314

    Purpose

    Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SpCas9-miniCGBE1

    Plasmid

    #198548

    Purpose

    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-miniCGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpCas9-NRRH

    Plasmid

    #198540

    Purpose

    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRRH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpCas9-NRCH

    Plasmid

    #198542

    Purpose

    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mycUb-20aa

    Plasmid

    #20255

    Depositor

    Nico Dantuma

    Article

    Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

    Insert

    Ubiquitin-20 amino acid extension (RBX1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-2TK-WW-CR-d-ZZ

    Plasmid

    #18071

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pGEX-2TK-CR-d-ZZ

    Plasmid

    #18075

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pGEX-2TK-WW-EF1+EF2

    Plasmid

    #18080

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pCDH-EF1-FLPe

    Plasmid

    #72262

    Purpose

    Express FLPe under EF-1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. N174-MCS

    Plasmid

    #81061

    Purpose

    Lentiviral vector for constitutive gene expression.

    Depositor

    Adam Karpf

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. N174-MCS (Puro)

    Plasmid

    #81068

    Purpose

    Lentiviral vector for constitutive gene expression.

    Depositor

    Adam Karpf

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAdx-EF1-tdTomato

    Plasmid

    #73354

    Purpose

    Express tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    EF1-tdTomato
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. plex_307-3xflag-ccdb-blasticidin

    Plasmid

    #201123

    Purpose

    pLEX_307 plasmid with N-terminal FLAG tag and blasticidin marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. plex_307-3xflag-ccdb-puro

    Plasmid

    #201122

    Purpose

    pLEX_307 plasmid with N-terminal FLAG tag and puro marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge