We narrowed to 3,042 results for: NAK

1. pCAG/ATP11C(E184Q)-HA

   Plasmid

   #209251

   Purpose

   Mammalian expression of ATP11C mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E184Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG/ATP8A1(E191Q)-HA

   Plasmid

   #209240

   Purpose

   Mammalian expression of ATP8A1 mutant E191Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP8A1 (ATP8A1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E191Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-OsTIR1(F74A)

   Plasmid

   #140731

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74A)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. ROSA26(MultiFPsΔPuro)

   Plasmid

   #140759

   Purpose

   Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Inserts

   puromycin-N-acetyltransferase
   Venus
   mCherry
   mCerulean
   

   Use

   Mouse Targeting

   Promoter

   CAGGS

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

   Plasmid

   #140535

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRL838

   Plasmid

   #70680

   Purpose

   This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

   Depositor

   Peter Wolk

   Article

   Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

   Type

   Empty backbone

   Use

   Cre/Lox

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-DIO-ECS-dSpyCatcher003

   Plasmid

   #234963

   Purpose

   Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   ECS-dSC
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   None

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-P3-IgKL-SpyCatcher003-GINKO2

   Plasmid

   #234544

   Purpose

   to express a potassium sensor tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-GINKO2
   

   Use

   AAV

   Mutation

   None

   Promoter

   P3

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-P3-IgKL-SpyCatcher003-SEP

   Plasmid

   #234541

   Purpose

   to express a pH sensor tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-SEP
   

   Use

   AAV

   Mutation

   None

   Promoter

   P3

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPAP_scFv_V50A

    Plasmid

    #183734

    Purpose

    Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Tags

    His6-tag

    Expression

    Yeast

    Mutation

    V50A mutation in MFalpha signal

    Promoter

    AOX1 promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMx/ATP8B1(E234Q)-HA

    Plasmid

    #209253

    Purpose

    Mammalian expression of ATP8B1 mutant E234Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B1 (ATP8B1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    E234Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG/ATP8B1(E234Q)-HA

    Plasmid

    #209241

    Purpose

    Mammalian expression of ATP8B1 mutant E234Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B1 (ATP8B1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E234Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IRES-puro-3xFLAG

    Plasmid

    #204545

    Purpose

    Expresses 3xFLAG tag in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    3xFlag
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-TetO(3G)-GCaMP6f-WPRE

    Plasmid

    #186205

    Purpose

    Express GCaMP6f under TetO(3G)

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Promoter

    TetO(3G)

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 8xNFAT-ZsG-hCD8

    Plasmid

    #153417

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK265 (RAD21-mAC Hygro)

    Plasmid

    #140539

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCold-I-Dsup

    Plasmid

    #90021

    Purpose

    To express His-tagged Dsup in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    cspA

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-miniDi-P2A-mCherry

    Plasmid

    #204358

    Purpose

    AAV vector for miniDi expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDi-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    The third intracellular loop (ICL3) of hM4Di was …

    Promoter

    hSyn

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid

    #162277

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

    Depositor

    Kazuhisa Nakayama

    Article

    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

    Insert

    HiFi Cas9
    

    Use

    CRISPR

    Tags

    BPNLS and FLAG tag

    Expression

    Mammalian

    Mutation

    SpCas9 (R691A)

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-miniDq-P2A-mCherry

    Plasmid

    #204357

    Purpose

    AAV vector for miniDq expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDq-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    The third intracellular loop (ICL3) of hM3Dq was …

    Promoter

    hSyn

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMRX-IRES-puro-3xFLAG-NBR1

    Plasmid

    #204546

    Purpose

    Expresses 3xFLAG-tagged NBR1 in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. mAID-BRD4 donor

    Plasmid

    #140650

    Purpose

    BRD4 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. CTCF-C CRISPR

    Plasmid

    #140647

    Purpose

    CTCF tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. BRD4-N CRISPR

    Plasmid

    #140651

    Purpose

    BRD4 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 8xNFAT-ZsG-muhCD4

    Plasmid

    #162745

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Human CD4 gene with glutamine to tyrosine at posi…

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDonor-tBFP-NLS-Neo (Universal)

    Plasmid

    #80767

    Purpose

    Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. CD3-2A_pMI-LO

    Plasmid

    #153418

    Purpose

    Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    murine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pENTR-DMD-Donor

    Plasmid

    #60605

    Purpose

    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    Hygromycin resistant gene
    

    Use

    Knock-in donor vector

    Expression

    Mammalian

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLKO.1-shDsup

    Plasmid

    #90024

    Purpose

    To establish Dsup knock down cells

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Expression

    Mammalian

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pPB-tetpA-hNEUROG2-iRPT

    Plasmid

    #140764

    Purpose

    Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

    Inserts

    iRFP713
    PuroR
    rtTAM2
    NEUROG2 (NEUROG2 Human)
    

    Expression

    Mammalian

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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