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pINS-Puro

Plasmid

#31389

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

pac

Use

Cre/Lox

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pGALT-EGFP-IRES-puro2b

Plasmid

#26463

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pGalT

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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PSUPER-1660(hPot1)

Plasmid

#11131

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi

Expression

Mammalian

Mutation

N/A

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
PM-ST1!TB

Plasmid

#48654

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-ST1!TA

Plasmid

#48653

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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eOYWP02

Plasmid

#238381

Purpose

phenazine gene for Golden Gate Assembly

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

phenazine operon

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 delta cerulean

Plasmid

#230939

Purpose

non-targeting Reference Control; expresses mCerulean

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 delta mcherry

Plasmid

#230940

Purpose

non-targeting Reference Control; expresses mCherry

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 fis cerulean

Plasmid

#230942

Purpose

aTc inducible knockdown of Fis

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 mcaS cerulean

Plasmid

#230944

Purpose

aTc inducible knockdown of mcaS

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 csgD cerulean

Plasmid

#230945

Purpose

aTc inducible knockdown of CsgD

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 flhDC cerulean

Plasmid

#230946

Purpose

aTc inducible knockdown of FlhDC

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 FliZ cerulean

Plasmid

#230947

Purpose

aTc inducible knockdown of FliZ

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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POC1553

Plasmid

#238470

Purpose

pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN13-Elen0616-Cber(ElDSM2243)

Plasmid

#225308

Purpose

Expresses Eggerthella lenta DSM 2243 Cber in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cber

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pUdOm3.1

Plasmid

#210283

Purpose

Empty backbone with chloramphenicol resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm4.1

Plasmid

#210284

Purpose

Empty backbone with chloramphenicol resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLNL1135

Plasmid

#160866

Purpose

PJ23110-RBS-CAT-taa-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Chloramphenicol Acetyltransferase

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23110_BBa_B0029

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAF302

Plasmid

#137752

Purpose

Intracellular control plasmid for HiBiTopt experiments

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

Insert

hupA-linker-hibitopt

Tags

HiBiTopt

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAP233

Plasmid

#137753

Purpose

Extracellular control for HiBiTopt experiments

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

Insert

srtB-linker-hibitopt

Use

Luciferase

Tags

HiBiTopt

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMXBC_pFa

Plasmid

#114249

Purpose

POC12391, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBC_aFb

Plasmid

#114250

Purpose

POC12392, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBC_bFc

Plasmid

#114251

Purpose

POC12393, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-c

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXBC_aFq

Plasmid

#114252

Purpose

POC12394, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXBC_bFq

Plasmid

#114253

Purpose

POC12395, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXBC_cFq

Plasmid

#114254

Purpose

POC12396, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type c-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXLC_aFb

Plasmid

#114220

Purpose

POC12356, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXLC_bFc

Plasmid

#114221

Purpose

POC12357, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-c

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge