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We narrowed to 4,533 results for: ARA-2

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Showing: 361 - 380 of 4533 results

  1. pfMDH_K381A

    Plasmid
    #67732
    Purpose
    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 381 to alanine
    Promoter
    T7
    Available Since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pfMDH_R373A

    Plasmid
    #67731
    Purpose
    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed arginine 373 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pSBL_DK186

    Plasmid
    #156422
    Purpose
    Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein
    Tags
    EGFP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156422-plasmid-map-sequence-id-306308

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  4. pSBL_DK198

    Plasmid
    #156425
    Purpose
    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    sfGFP-His
    Expression
    Insect
    Mutation
    aa 42-275
    Promoter
    Polyhedrin
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156425-plasmid-map-sequence-id-307748

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  11. pHis-TEV-Nsp15-E266Q

    Plasmid
    #218803
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    E266Q (G to C)
    Available Since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218803-plasmid-map-sequence-id-433302

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  12. pHis-TEV-Nsp15-V66L

    Plasmid
    #219524
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    V66L (G to C)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219524-plasmid-map-sequence-id-433377

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  13. pHis-TEV-Nsp15-A81V

    Plasmid
    #219525
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    A81V (C to T)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219525-plasmid-map-sequence-id-433375

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  14. pHis-TEV-Nsp15-D183N

    Plasmid
    #219526
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    D183N (G to A)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219526-plasmid-map-sequence-id-433305

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  15. pHis-TEV-Nsp15-H234A

    Plasmid
    #219529
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    H234A (CAC to GCG)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219529-plasmid-map-sequence-id-433314

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  16. pSBL_DK202

    Plasmid
    #156426
    Purpose
    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    aa 42-275
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156426-plasmid-map-sequence-id-306303

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  18. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available Since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pGTM_Sumo2-C-8XHis-PLpro

    Plasmid
    #234321
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage an 8xHis Tag is retained at the C-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234321-plasmid-map-sequence-id-469248

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Showing: 361 - 380 of 4533 results