We narrowed to 1,015 results for: HTA

Showing: 361 - 380 of 1015 results

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pcDNA3.1(+)-RIα-EGFP

Plasmid

#181840

Purpose

GFP-tagged PKA RIα subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIIα-Dronpa

Plasmid

#182331

Purpose

PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

RIIalpha-Dronpa (PRKAR2A Human)

Tags

Full-length human PKA type 2alpha regulatory subu…

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-Luciferase-CD4-bla

Plasmid

#189981

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP11(x7)-Actin

Plasmid

#181967

Purpose

Human beta actin tagged with 7 copies of GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11(x7)-Actin (ACTB )

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-FRT

Plasmid

#113398

Purpose

expresses gRNA for Cas9 FRT targetting

Depositor

Jan Michiels

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

exo, beta, gam, sgRNA-FRT

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmExRai-AKAR2

Plasmid

#161755

Purpose

Plasma membrane-targeted ExRai-AKAR2.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

Lyn-ExRai-AKAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDuEx2-NIR-GECO1

Plasmid

#113680

Purpose

Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

Depositor

Robert Campbell

Article

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NIR-GECO1

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloCKAR2.2-T2A-EGFP

Plasmid

#216493

Purpose

Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloCKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-Digda

Plasmid

#236102

Purpose

Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Lyso-Digda (LAMP1 Synthetic, Human)

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mitoExRai-AKAR2

Plasmid

#161757

Purpose

ExRai-AKAR2 targeted to the outer mitochondrial membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

DAKAP-ExRai-AKAR2

Tags

N-terminal 30 amino acids from DAKAP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2(T/A)

Plasmid

#161754

Purpose

Negative-control mutant for ExRai-AKAR2 biosensor.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2(T/A)

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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TccCas13a Twin SUMO

Plasmid

#199754

Purpose

Expresses codon optimized thermostabile TccCas13a in E.coli

Depositor

Magdy Mahfouz

Article

Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

TccCas13a

Tags

SUMO

Expression

Bacterial

Promoter

LacI

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-erExRai-AKAR2

Plasmid

#161756

Purpose

ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

CYP450-ExRai-AKAR2

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Actin

Plasmid

#181966

Purpose

Human beta actin tagged with GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-Actin (ACTB Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-CreER

Plasmid

#129093

Purpose

KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

CreERT2 and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

Plasmid

#216491

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

NES, T2A-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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