We narrowed to 1,041 results for: HTA
-
Plasmid#181856PurposeGenetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AktAR
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianPromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10
Plasmid#181859PurposePart of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.DepositorInsertcp-PKN-mRuby2-GFP(1-0)
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-SRF
Plasmid#98618PurposeGateway entry TOPO TA cloning vector for SRFDepositorInsertSRF (Srf Mouse)
UseTopo ta cloning vectorAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionPromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2
Plasmid#48082PurposeMammalian expression of Gal4 tagged MeCP2-WTDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
ExpressionMammalianAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 L756A/I757A
Plasmid#19938DepositorInsertcullin 1 L756A/I757A (CUL1 Human)
TagsHA2ExpressionMammalianMutationMutation of two adjacent hydrophobic residues, L…Available SinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
Tags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianPromoterCMVAvailable SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsub(TA)DD-Venus-NES
Plasmid#84631PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsub(TA)DD-Venus-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationMutated target Thr residue to Ala.PromoterCMVAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-2B-Nkx2-5
Plasmid#98612PurposeGateway entry vector for NKX2-5DepositorInsertNKX2-5 (Nkx2-5 Mouse)
UseEntry vectorAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianPromoterCMVAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet02
Plasmid#78284PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(S431A)
Plasmid#84641PurposemCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation siteDepositorInsertmCherry-LBK1(S431A) (STK11 Synthetic, Human)
Tags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPKA phosphorylation site Serine 428 mutated to Al…PromoterCMVAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403) L361C
Plasmid#213622PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403) L361CDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
TagsSUMOExpressionBacterialMutationY96C, C139A, C141SAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
Tags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
TagsCFP(1-10) and cpVenus(E127)ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP11(x7)-Actin
Plasmid#181967PurposeHuman beta actin tagged with 7 copies of GFP11.DepositorInsertGFP11(x7)-Actin (ACTB )
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-Digda
Plasmid#236102PurposeLysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.DepositorAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only