We narrowed to 3,282 results for: cgas

1. CBX1 A3.5 gRNA

   Plasmid

   #90566

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CBX1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CBX1 (Guide Designation A3.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDY2259 hNOLC1 atgRNA Paired Guide 1

   Plasmid

   #220989

   Purpose

   hNOLC1 atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDY2260 hNOLC1 atgRNA Paired Guide 2

   Plasmid

   #220990

   Purpose

   hNOLC1 atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDY2261 hACTB atgRNA Paired Guide 1

   Plasmid

   #220991

   Purpose

   hACTB atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDY2262 hACTB atgRNA Paired Guide 2

   Plasmid

   #220992

   Purpose

   hACTB atgRNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO.1-Teton-puro-shPP1C #1

   Plasmid

   #198762

   Purpose

   conditional knockdown of PP1C

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

   Insert

   shPP1C #1 (PPP1CC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Circular 200,100 with interspersed loops (FANCC)

   Plasmid

   #180192

   Purpose

   AAV vector carrying a guide RNA targeting the human FANCC mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

   Plasmid

   #217345

   Purpose

   crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
   crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
   crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
   crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
   crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
   crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
   crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
   crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
   crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
   crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX459-HypaCas9-ACTB_sgRNA

    Plasmid

    #183885

    Purpose

    pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCHKO_TK1_HPRT1_Lb_1

    Plasmid

    #155059

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAP14

    Plasmid

    #186262

    Purpose

    wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    wgaAB bicistronic
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-sgRnf43#1/Cre

    Plasmid

    #173613

    Purpose

    Expresses a Rnf43-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Rnf43 (Rnf43 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Circular 200,100 (GAPDH)

    Plasmid

    #170120

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid

    #183884

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCHKO_TK1_HPRT1_Lb_4

    Plasmid

    #155062

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PRKAR2A gRNA (BRDN0001145818)

    Plasmid

    #77672

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR2A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR2A (PRKAR2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1-mTagBFP2-rMPC1 shRNA

    Plasmid

    #229016

    Purpose

    Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Rat)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid

    #155064

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCHKO_TK1_HPRT1_Lb_5

    Plasmid

    #155063

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge