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Plasmid#183572PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)DepositorInsertAttenuator Sequence 5
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-EF1a.3
Plasmid#183570PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)DepositorInsertAttenuator Sequence 3
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-EF1a.1
Plasmid#183568PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)DepositorInsertAttenuator Sequence 1
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTEF1-UDH
Plasmid#166679PurposeYeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertUDH
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1n2_mFAP2b
Plasmid#159471PurposeEncodes for EF1n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1n2_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1n3_mFAP2b
Plasmid#159472PurposeEncodes for EF1n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1n3_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1n_mFAP2b
Plasmid#159473PurposeEncodes for EF1n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1n_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD
Plasmid#162814PurposeSTELLA plasmid for amber suppression of C-terminally labelled SMIM38DepositorInsertSMIM38(C-TAG)-Intein-DD
UsePiggybacTagsExpressionMammalianMutationSMIM38(Ochre52,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_M
Plasmid#162815PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_MDepositorInsertUb-(N-TAG)CoV2_M
UsePiggybacTagsExpressionMammalianMutationCoV2_M(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b
Plasmid#162816PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_9bDepositorInsertUb-(N-TAG)CoV2_9b
UsePiggybacTagsExpressionMammalianMutationCoV2_9b(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6
Plasmid#162817PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6DepositorInsertUb-(N-TAG)CoV2_ORF6
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF6(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b
Plasmid#162818PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7bDepositorInsertUb-(N-TAG)CoV2_ORF7b
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF7b(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10
Plasmid#162819PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10DepositorInsertUb-(N-TAG)CoV2_ORF10
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF10(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14
Plasmid#162820PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14DepositorInsertUb-(N-TAG)CoV2_ORF9c/14
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF9c/14(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt
Plasmid#162821PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_altDepositorInsertUb-(N-TAG)CoV2_ORF3b_alt
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF3b_alt(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*SMIM38
Plasmid#162808PurposeSTELLA plasmid for amber suppression of N-terminally labelled SMIM38DepositorInsertUb-(N-TAG)SMIM38
UsePiggybacTagsExpressionMammalianMutationSMIM38 (Met1, Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4x7SKPylT_EF1_Ub-*PIGBOS
Plasmid#162809PurposeSTELLA plasmid for amber suppression of N-terminally labelled PIGBOSDepositorInsertUb-(N-TAG)PIGBOS
UsePiggybacTagsExpressionMammalianMutationPIGBOS (Met1, Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A2_GTP-EFTU-D2
Plasmid#109919PurposeProtein expression and purification of EEF1A2_GTP-EFTU-D2DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L5-EF1_-R3
Plasmid#141037PurposeMutisite Gateway mediated vector constructionDepositorInsertEF1_ promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-GpNLuc
Plasmid#135949PurposeExpresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only