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pEGFP-Rab21-Q76L (CA)

Plasmid

#83422

Purpose

Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residue 76 from Q to L (Q76L: CAG -> CTG)

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Fyn

Plasmid

#16033

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Dominant negative: K299M

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
mEos2-Actin-7

Plasmid

#57339

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Actin (ACTB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGL3-ITGA6-1k-Basic-LUC

Plasmid

#107141

Purpose

Luciferase reporter plasmid to monitor transcriptional activity of canine ITGA6-promoter (1kb fragment)

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

ITGA6-promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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Pinco-mNrp1

Plasmid

#21937

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
DNM2 delta PRD

Plasmid

#168275

Purpose

Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

DNM2 (DNM2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa 1-742

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-CCSS

Plasmid

#83424

Purpose

Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

Promoter

CMV

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-RIAM(1-666)

Plasmid

#80028

Purpose

Mammalian expression of GFP-RIAM(1-666)

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

RIAM (APBB1IP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-666

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
dsRedm-Rab21

Plasmid

#83425

Purpose

Full-length mouse Rab21 (wildtype)

Depositor

Johanna Ivaska

Article

Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

Insert

Rab21 (Rab21 Mouse)

Use

Tags

dsRed-monomer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Bin1 delta N-BAR

Plasmid

#168282

Purpose

Express N-BAR-deleted Bin1 mutant (aa 268-476)

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

aa 268-476

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Pinco-mNrp1dCy

Plasmid

#21936

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

deleted amino acids 884-923

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
Pinco-mNrp1dSEA

Plasmid

#21935

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

deleted amino acids 920-923

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
Bin1 delta SH3

Plasmid

#168283

Purpose

Express SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-404

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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FN207

Plasmid

#50497

Depositor

Kenneth Yamada

Article

Danen et al J Biol Chem. 1995 Sep 15;270(37):21612-8

Insert

NM_001306129.1 (FN1 Human)

Use

Tags

Expression

Bacterial

Mutation

repeats 6-10

Promoter

T7

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 (+) Tac-delta scr

Plasmid

#80019

Purpose

Mammalian expression of Tac-delta scr

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tac (IL2RA Human)

Use

Tags

KLRFGFK

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLRFGFK

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pEF.DEST51-PTPN12(D199A,C231S)-Clover

Plasmid

#215528

Purpose

Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Tags

Clover

Expression

Mammalian

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-Dok1-mScarlet

Plasmid

#215535

Purpose

Expression vector with Dok1 tagged with C-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12(D199A,C231S)

Plasmid

#215518

Purpose

Dox-inducible lentiviral transfer vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Tags

Expression

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

mCMV, Doxycycline-inducible

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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