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dora[B]_rescue_construct

Plasmid

#190637

Purpose

Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-Laccase2 608-1097

Plasmid

#69890

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Insect

Mutation

L158I in laccase2

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pUASp-Flag-Myc-Qin-Tudor-attB

Plasmid

#44210

Depositor

Phillip Zamore

Article

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin-Tudor-domain (qin Fly)

Use

Fly transformation

Tags

Flag and Myc

Mutation

lacks E3 domain on N-term (del aa1-274)

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00676

Plasmid

#39724

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG42277 (rn Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00156

Plasmid

#34310

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG16899 (FoxP Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUAST attB Orco (Y478A)

Plasmid

#73928

Purpose

UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

Orco (Y478A) (Orco Fly)

Use

Unspecified

Mutation

changed 478 Tyrosine for Alanine

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
pTYF-PRSx8-AstR-p2A-GFP

Plasmid

#159632

Purpose

Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

Depositor

Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

AstR (AstA-R1 Fly)

Use

Lentiviral

Tags

GFP and P2A

Expression

Mammalian

Promoter

PRSx8

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 Exons 1-3

Plasmid

#91799

Purpose

Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

Laccase2 (stw Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS6 AA 1035-1284

Plasmid

#196904

Purpose

Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 6 (IntS6 Fly)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS11 AA 300-597

Plasmid

#199329

Purpose

Expresses His-tagged Drosophila IntS11 amino acids 300-597

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 11 (IntS11 Fly)

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 300-597 only

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

Plasmid

#69899

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (stw Fly, Human)

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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KHBD00019

Plasmid

#34181

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5461 (bun Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmNot1_J

Plasmid

#146709

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Expression

Insect

Mutation

Three non silent mutations N605S, K759M, G1999S a…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_CG6867_nostop

Plasmid

#240238

Purpose

Gateway entry clone with CG6867 without stop codon

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMtnA FLAG-IntS13 puro

Plasmid

#208406

Purpose

Expresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS13 (asun Fly)

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMtnA FLAG-IntS14 puro

Plasmid

#208407

Purpose

Expresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS14 (IntS14 Fly)

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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