We narrowed to 1,583 results for: pbluescript

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pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

Plasmid

#87260

Purpose

YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.

Depositor

David Sibley

Article

Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

Inserts

YFP-AID-3HA
HXGPRT

Use

Toxoplasma expression

Tags

3HA and AID

Promoter

TgDHFR-TS 463 bp and TgTUB1 378 bp

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBS_flGAD

Plasmid

#87031

Purpose

Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria

Depositor

Janice Blum

Insert

flGAD (GAD2 Human)

Expression

Bacterial

Promoter

Lac-Z

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pY2F

Plasmid

#53285

Purpose

Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Expression

Bacterial

Promoter

lac

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHyVec1

Plasmid

#34789

Depositor

Rob Steele

Type

Empty backbone

Use

Hydra expression

Tags

Actin/GFP fusion protein

Promoter

Hydra vulgaris actin promoter

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only

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zWnt5b

Plasmid

#21282

Depositor

Randall Moon

Article

Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

Insert

Wnt5b (wnt5b Zebrafish)

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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pBSK-SN3 (wt p53)

Plasmid

#16421

Depositor

Bert Vogelstein

Article

Baker et al Science. 1989 Apr 14. 244(4901):217-21.

Insert

p53 (TP53 Human)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
Pdx1-RFP hygro (DM#236)

Plasmid

#15110

Depositor

Douglas Melton

Article

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Tags

RFP

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pBS CRX (CC#4)

Plasmid

#15127

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Expression

Bacterial

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
Chx10 3kb AP (CC#318)

Plasmid

#15205

Depositor

Connie Cepko

Article

Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Insert

Chx10 3kb AP reporter (Vsx2 Mouse)

Expression

Bacterial

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
3185 pBS Bax cDNA

Plasmid

#8754

Depositor

Stanley Korsmeyer

Article

Knudson et al Science 1995 Oct 6;270(5233):96-9.

Insert

Bax (Bax Mouse)

Expression

Bacterial

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
Gata3-T2A-H2B-eGFP donor

Plasmid

#113119

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata3 donor region including homology arms and T2A-H2B-eGFP

Use

Synthetic Biology

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Ctcf-Halo-mAID donor

Plasmid

#113103

Purpose

Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Synthetic, Mouse)

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRosa26-4xInsulator (JP6)

Plasmid

#99627

Purpose

IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJC30X-BS(X)

Plasmid

#58958

Purpose

Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

Depositor

Thomas Fox

Article

Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.

Insert

Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code

Tags

First 8 codons of COX3

Mutation

GFP modified as described: CORMACK, B. P., R. H. …

Promoter

n/a

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
0-Luc

Plasmid

#21723

Depositor

Wafik El-Deiry

Article

Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.

Insert

p21 promoter deletion mutant (CDKN1A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
SEMA3A (mouse) pBLUE

Plasmid

#29450

Depositor

Jonathan Raper

Insert

SEMA3A (Sema3a Mouse)

Use

For ish

Mutation

G25R, S67R and Y124C confirmed

Available Since

Feb. 24, 2012

Availability

Academic Institutions and Nonprofits only

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mSprouty4 EST

Plasmid

#22093

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 4 (Spry4 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only