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We narrowed to 9,067 results for: sgRNA

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Showing: 361 - 380 of 9067 results

  3. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Publication
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112139-plasmid-map-sequence-id-217823

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  6. pSmart-Nm-sgRNA-BbsI

    Plasmid
    #49157
    Purpose
    Plasmid for cloning spacer into sgRNA for NmCas9
    Depositor
    James Thomson
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    49157-plasmid-map-sequence-id-72446

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  7. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  11. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71286-plasmid-map-sequence-id-127777

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  12. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Available since
    Oct. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159752-plasmid-map-sequence-id-313824

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  16. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

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  17. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid
    #154197
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Publication
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC2-A, sgRNA-BC2-C
    Use
    Lentiviral; Catch barcode activation
    Expression
    Mammalian
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154197-plasmid-map-sequence-id-308682

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  19. pX330 _CRISPIE donor B7

    Plasmid
    #172848
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172848-plasmid-map-sequence-id-341024

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Showing: 361 - 380 of 9067 results