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We narrowed to 6,195 results for: cas9 expression plasmid

Showing: 5671 - 5700 of 6195 results

  1. pGG438

    Plasmid
    #165486
    Purpose
    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, S1136R, D1332K, and R1333G mutations in S…
    Promoter
    CMV and T7
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165486-plasmid-map-sequence-id-330963

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  3. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Article
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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  4. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  5. pETM6-3F2-mCherry

    Plasmid
    #73418
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3F2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73418-plasmid-map-sequence-id-135318

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  6. pETM6-3A2-mCherry

    Plasmid
    #73425
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3A2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3A2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73425-plasmid-map-sequence-id-135325

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  7. pETM6-5F5-mCherry

    Plasmid
    #73422
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 5F5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P5F5 (orthogonal T7-lac variant)
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73422-plasmid-map-sequence-id-135322

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  8. pETM6-1D4-mCherry

    Plasmid
    #73423
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1D4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1D4 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73423-plasmid-map-sequence-id-135323

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  9. pETM6-4A6-mCherry

    Plasmid
    #73424
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4A6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4A6 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73424-plasmid-map-sequence-id-135324

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  10. pETM6-1E4-mCherry

    Plasmid
    #73426
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1E4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1E4 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73426-plasmid-map-sequence-id-135326

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  11. pETM6-1B6-mCherry

    Plasmid
    #73420
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1B6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1B6 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73420-plasmid-map-sequence-id-135320

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  12. pETM6-3H5-mCherry

    Plasmid
    #73419
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3H5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3H5 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73419-plasmid-map-sequence-id-135319

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  15. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

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  16. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  18. pWPT-/mEGFP-1T-IRES-mCherry

    Plasmid
    #190190
    Purpose
    Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.
    Depositor
    Alessandro Quattrone
    Article
    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    changed EGFP Kozak sequence inserting a C>T mo…
    Promoter
    EIF1-short
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    190190-plasmid-map-sequence-id-375199

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  26. SunTagCACTA1g2-22aa-TET1cd

    Plasmid
    #106437
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106437-plasmid-map-sequence-id-205837

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  27. pKS7107

    Plasmid
    #89051
    Purpose
    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
    Depositor
    Ervin Welker
    Article
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Inserts
    Nm crRNA
    Nm tracrRNA
    hNmCas9
    Use
    CRISPR
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89051-plasmid-map-sequence-id-176334

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Showing: 5671 - 5700 of 6195 results