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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 9451 - 9500 of 21628 results

  2. pTEI127

    Plasmid
    #126067
    Purpose
    Module 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Insert
    mitochondria Rieske iron sulphur protein (1-100 aa)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    'long' CaMV35S
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126067-plasmid-map-sequence-id-246127

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  19. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119697-plasmid-map-sequence-id-232493

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  24. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

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  25. pTH846-gst-dissc

    Plasmid
    #96907
    Purpose
    Plasmid for yeast expression of GST encoded by non-preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon dis-optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96907-plasmid-map-sequence-id-206054

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  26. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

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  27. pTH858-gst-optec V156E

    Plasmid
    #89592
    Purpose
    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed valine 156 to glutamic acid
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89592-plasmid-map-sequence-id-206041

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  28. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

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  42. pVH004

    Plasmid
    #80401
    Purpose
    Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    CusR (cusR )
    AraC (araC )
    Anti-scaffold
    Use
    Synthetic Biology
    Tags
    C-terminal LZx domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80401-plasmid-map-sequence-id-155911

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  47. Nabi2.30

    Plasmid
    #73798
    Purpose
    Nabi2.30 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.30
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73798-plasmid-map-sequence-id-136837

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  48. Nabi2.104

    Plasmid
    #73799
    Purpose
    Nabi2.104 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.104
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73799-plasmid-map-sequence-id-136838

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  49. Nabi2.125

    Plasmid
    #73800
    Purpose
    Nabi2.125 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.125
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73800-plasmid-map-sequence-id-136839

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  50. Nabi2.216

    Plasmid
    #73801
    Purpose
    Nabi2.216 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.216
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73801-plasmid-map-sequence-id-136840

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Showing: 9451 - 9500 of 21628 results