We narrowed to 8,772 results for: App

1. pMC7-XI1dw

   Plasmid

   #176167

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-1
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMC8b-XI2up

   Plasmid

   #176168

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XI-2
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC7-XI2dw

   Plasmid

   #176169

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-2
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMC8b-XII2up

   Plasmid

   #176174

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XII-2
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMC7-XII2dw

   Plasmid

   #176175

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XII-2
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC8b-X4up

   Plasmid

   #176164

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site X-4
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMC7-X4dw

   Plasmid

   #176165

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site X-4
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJL30

   Plasmid

   #174064

   Purpose

   Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.

   Depositor

   Albert Keung

   Article

   Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

   Insert

   pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   pGal1

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

   Plasmid

   #176573

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176577

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbdCas9S_P0526-sgRNArodA

    Plasmid

    #149658

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL15

    Plasmid

    #174069

    Purpose

    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    pGal1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBluescript-Q22YU3-KO

    Plasmid

    #170313

    Purpose

    Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 knockout
    

    Use

    Tetrahymena somatic gene knockout vector

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBluescript-Q22MS1-KO

    Plasmid

    #170312

    Purpose

    Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22MS1 knockout
    

    Use

    Tetrahymena somatic gene knockout vector

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACEBac1-Q22MS1-2xStrep

    Plasmid

    #170314

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22MS1
    

    Tags

    Strep-Tag II

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-EGFP-DmMe31B_268-459_D

    Plasmid

    #146119

    Purpose

    Insect Expression of DmMe31B_268-459

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmMe31B_268-459 (me31B Fly)
    

    Expression

    Insect

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. IBMc120

    Plasmid

    #161950

    Purpose

    Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB4A3-PECF20-B30-mScarlet-I-B15
    

    Expression

    Bacterial

    Promoter

    PECF20

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. IBMc393

    Plasmid

    #161953

    Purpose

    Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB4A3-T-PT7-B30-mScarlet-I-3T
    

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IBMc330

    Plasmid

    #161952

    Purpose

    Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA171-PSrpR-B30-mScarlet-I-Ter
    

    Expression

    Bacterial

    Promoter

    PSrpR

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. IBMc050

    Plasmid

    #161938

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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