We narrowed to 26,489 results for: CAL;

1. eDrMet

   Plasmid

   #183999

   Purpose

   Optically controlled eDrcMet expression in mammalian cells

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrMet (MET Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLD-CAG-DYSF-CPL

   Plasmid

   #131458

   Purpose

   Encodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

   Depositor

   Michele Calos

   Article

   Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

   Insert

   DYSF (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. iDianiSnFR

   Plasmid

   #177741

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iDianiSnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. iCytSnFR

   Plasmid

   #177738

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCytSnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. iCyt_BrEt_SnFR

   Plasmid

   #177740

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCyt_BrEt_SnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. iCyt_F_SnFR

   Plasmid

   #177739

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCyt_F_SnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX

   Plasmid

   #176121

   Purpose

   Constitutivley active, mitochondrial localized Cdc42

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

   Insert

   CDC42 (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

   Plasmid

   #176123

   Purpose

   Constitutively active, mitochondrial localized RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

   Insert

   RHOA (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G14V

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEntr1a-SspB-HaloTag-TIAM1-DHPH

   Plasmid

   #176134

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS405 SEC66-VAC8-GFP

    Plasmid

    #166840

    Purpose

    Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, SEC66 Budding Yeast)
    

    Use

    Tags

    GFP and SEC66 transmembrane domain

    Expression

    Yeast

    Mutation

    SEC66 transmembrane domain

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

    Plasmid

    #164132

    Purpose

    Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    ciCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBTetDBl-CLIP-Centrin2

    Plasmid

    #136834

    Purpose

    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin2 (CETN2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBTetBl-CLIP-Centrin2

    Plasmid

    #136833

    Purpose

    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin2 (CETN2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

    Plasmid

    #83458

    Purpose

    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4127-4303, S4144D

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mEos2-Farnesyl-5

    Plasmid

    #57375

    Purpose

    Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Catherine Galbraith

    Article

    Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. PA-mCherry-miniSOG-H2B-C-10

    Plasmid

    #57789

    Purpose

    Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. psicheck-2-Casp8-mod

    Plasmid

    #48171

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> ATT) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of CASP8 (CASP8 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    G/T MUTATION IN ATG OF UORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

18. psicheck-2-Mc2r-mod

    Plasmid

    #48173

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of MC2R (MC2R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/G MUTATION IN ATG OF UORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

19. psicheck-2-Kdr-mod

    Plasmid

    #48177

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of 2KDR (MC2R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/G MUTATION IN ATG OF UORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

20. psicheck-2-Hsdl2-mod

    Plasmid

    #48180

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of HSDL2 (Hsdl2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/T MUTATION IN ATG OF UORF

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only