We narrowed to 29,515 results for: REP

1. eGFP-KIF1A

   Plasmid

   #172206

   Purpose

   Expresses KIF1A tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF1A (Kif1a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LT3GEPIR

   Plasmid

   #111177

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBR_attB(bxb)_lox

   Plasmid

   #183762

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SGEP

   Plasmid

   #111170

   Purpose

   miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   SFFV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1-1x

   Plasmid

   #172281

   Purpose

   Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

   Depositor

   Antony K Chen

   Article

   Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

   Insert

   EGFP-N1-1x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAP2

   Plasmid

   #73211

   Purpose

   express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   AAP2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-NLS-CBGreen-FIRE-puro

   Plasmid

   #139195

   Purpose

   Luminescent reporter of ERK activity

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBG99
   

   Use

   Luciferase and Retroviral

   Tags

   Fra1 PEST domain fragment (FIRE) and nuclear loca…

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. sUPRa

    Plasmid

    #223242

    Purpose

    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

    Depositor

    Colin Akerman

    Article

    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

    Insert

    sUPRa
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Custom

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Green Lindoblum

    Plasmid

    #163113

    Purpose

    Expresses biosensor for lactate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Lindolum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1_DNAseI

    Plasmid

    #182408

    Purpose

    Bacterial expression of DNaseI

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-2XFL-wtSrc

    Plasmid

    #140295

    Purpose

    Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src wildtype (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST40-2XHA-wtSrc

    Plasmid

    #140294

    Purpose

    Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src wildtype (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBbsr2-4031NES

    Plasmid

    #105241

    Purpose

    A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    AMPK-EV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-mPKR-FLAG-IG

    Plasmid

    #101792

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse PKR

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Pkr (Eif2ak2 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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