We narrowed to 21,813 results for: Sis

1. pWZLblasti-HA-MEK1-MEK5DD

   Plasmid

   #53177

   Purpose

   Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   MEK1 (1-201) MEK5(498–1344 with S311D/T315D)

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pLV-mEGFP-hCTNNA1_DM1-3_WT-ABD

   Plasmid

   #234553

   Purpose

   CTNNA1 M-domain deletion mutant

   Depositor

   Cara Gottardi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

   Insert

   mEGFP:hCTNNA1_DM-domain (CTNNA1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Deletion of aa 273-635

   Promoter

   CMV

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-mEGFP-hCTNNA1_L436P

   Plasmid

   #234559

   Purpose

   CTNNA1 eye phenotype via forward genetic screen

   Depositor

   Cara Gottardi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

   Insert

   mEGFP:hCTNNA1_L436P (CTNNA1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   L436P

   Promoter

   CMV

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-mEGFP-hCTNNA1_DNii domain

   Plasmid

   #234557

   Purpose

   CTNNA1 Nii-domain deletion mutant

   Depositor

   Cara Gottardi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

   Insert

   mEGFP:hCTNNA1_DNii domain (CTNNA1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Deletion of aa ~165-265

   Promoter

   CMV

   Available since

   June 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234370

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234372

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-24XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMVP-SEC24C

   Plasmid

   #231559

   Purpose

   pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)

   Depositor

   Rizwan Haq

   Article

   Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

   Insert

   SEC24C (SEC24C Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   silent PAM mutations (G67 (GGG>GGA) and A115 (…

   Promoter

   CMV

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

   Plasmid

   #231007

   Purpose

   Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

   Insert

   E4F1 aa220-242 (E4F1 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-CAPN7-C290S-mCherry

   Plasmid

   #193047

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hTph2p-Luc

    Plasmid

    #223526

    Purpose

    Firefly-luciferase reporter expression driven by human Tph2 promoter

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    Tph2 promoter (TPH2 Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    hTph2 promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-mCherry-KATNA1, V55D

    Plasmid

    #180651

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. B52_puro_gRNA_3418711_KCNB2

    Plasmid

    #197558

    Purpose

    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

    Depositor

    Darius Bägli

    Insert

    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    

    Use

    Grna with puromycin selection

    Tags

    puromycin resistance cassette under CMV promotion

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Coexpressed Free NES

    Plasmid

    #182482

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dCas9_sgRNA Td2

    Plasmid

    #176258

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNOC_dfnCas12a_crRNA Td2

    Plasmid

    #176255

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4

    Plasmid

    #175815

    Purpose

    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller, Mark Banfield

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Expression

    Mutation

    Promoter

    none

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2

    Plasmid

    #175816

    Purpose

    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller, Mark Banfield

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Expression

    Mutation

    natural polymorphism P65S, annotated in Genbank f…

    Promoter

    none

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 9

    Plasmid

    #175817

    Purpose

    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 9 (AT5G03070.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller, Mark Banfield

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 9 (IMPA-9 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Expression

    Mutation

    Promoter

    none

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid

    #153441

    Purpose

    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc. XDocB binding mode B knock-out mutant
    

    Use

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Arginine 140 and methionine 144 were mutated to a…

    Promoter

    T7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid

    #176262

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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