We narrowed to 10,047 results for: yeast

1. pAL003

   Plasmid

   #233864

   Purpose

   MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYO225

   Plasmid

   #235729

   Purpose

   Protin expression of FL yeast VPS15 -ZZ in yeast

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS15
   

   Use

   Tags

   3xTEV-ZZ

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYO290

   Plasmid

   #235731

   Purpose

   Protein expression in FL yeast VPS34 D731N mutant in yeast

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS34
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   S2A, D731N

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCY5h

   Plasmid

   #160295

   Purpose

   Yeast CRISPR plasmid targeting the hphMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCY5n

   Plasmid

   #160296

   Purpose

   Yeast CRISPR plasmid targeting the natMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCY5k

   Plasmid

   #160294

   Purpose

   Yeast CRISPR plasmid targeting the kanMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9-EcRT-Z3-Nat

   Plasmid

   #232094

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWR58

   Plasmid

   #202795

   Purpose

   integrating bioluminescene reporter for Cas9 activity in S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   P-TDH3/coCBG(first portion)-YUM1
   P-TEF1/ShBle/T-ACT1
   YUM1-coCBG(last portion)/T-ADH2
   URA3 and flanking sequence
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIV272

   Plasmid

   #226183

   Purpose

   Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1p (Komagataella phaffii)

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKanB

    Plasmid

    #223523

    Purpose

    Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHYX143

    Plasmid

    #202816

    Purpose

    Control plasmid expressing mScarlet-I fluorescent protein.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPCK1

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHYX173

    Plasmid

    #202817

    Purpose

    Plasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Insert

    TEV protease
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPCK1

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGADT7-AP-4 β4

    Plasmid

    #198327

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-4 β4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Contains L480S substitution

    Promoter

    ADH1

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGADT7-AP-1 σ1C

    Plasmid

    #198336

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1C
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    S148C substitution

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACT2-AP-3 σ3A

    Plasmid

    #198347

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-3 σ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACT2-AP-3 σ3B

    Plasmid

    #198348

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-3 σ3B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCL.005

    Plasmid

    #184971

    Purpose

    Express Cas9 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Integrating inducible cassette: Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB9339 (pgRNA_VII-1_NatMX)

    Plasmid

    #161591

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB9340 (pgRNA_VIII-1_NatMX)

    Plasmid

    #161592

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB9341 (pgRNA_IX-1_NatMX)

    Plasmid

    #161593

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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