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Showing: 7601 - 7640 of 8645 results

  2. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid
    #236041
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236041-plasmid-map-sequence-id-469952

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  3. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid
    #236039
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236039-plasmid-map-sequence-id-469911

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  7. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

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  9. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  21. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid
    #213401
    Purpose
    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    Expression
    Plant
    Promoter
    2x35S
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213401-plasmid-map-sequence-id-422351

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  26. pGA-red-mini

    Plasmid
    #196338
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196338-plasmid-map-sequence-id-393164

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  27. pGA-red-maxi

    Plasmid
    #196337
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196337-plasmid-map-sequence-id-393160

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  28. pSCL.178

    Plasmid
    #184999
    Purpose
    Express -Eco1 FANCF editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    FANCF donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184999-plasmid-map-sequence-id-376704

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  29. pSCL.176

    Plasmid
    #184997
    Purpose
    Express -Eco1 RNF2 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    RNF2 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184997-plasmid-map-sequence-id-376646

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  30. pSCL.179

    Plasmid
    #185000
    Purpose
    Express -Eco1 HEK4 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK4 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    185000-plasmid-map-sequence-id-376622

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  35. pSBK.136

    Plasmid
    #187220
    Purpose
    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively
    Depositor
    Seth Shipman
    Article
    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:
    Insert
    Retron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    Expression
    Bacterial
    Promoter
    A: pSalTTC; B: pTet*
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187220-plasmid-map-sequence-id-368082

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Showing: 7601 - 7640 of 8645 results