We narrowed to 14,583 results for: pET

1. pET28b(+)_S. cerevisiae Hbs1(162-611)

   Plasmid

   #39337

   Depositor

   SeWon Suh

   Insert

   Elongation factor 1 alpha-like protein
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pET-21a(+)-DR RecR(K23A/R27A)

   Plasmid

   #12619

   Depositor

   SeWon Suh

   Article

   Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

   Insert

   Deinococcus radiodurans RecR (recR Deinococcus radiodurans)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Lysine 23 to Alanine, and Arginine 27 to …

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. p141 pETF-GST RanBP10 G308R

   Plasmid

   #11094

   Depositor

   Ramesh Shivdasani

   Insert

   RanBP10 (Ranbp10 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G308R

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

   Plasmid

   #117981

   Purpose

   SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B1
   

   Use

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

   Plasmid

   #117982

   Purpose

   SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B2
   

   Use

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

   Plasmid

   #117983

   Purpose

   SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B2
   

   Use

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. Kv7.4 BD (522-645, C643A)/pET28HMT

   Plasmid

   #111073

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 BD (522-645, C643A)

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pet28a His6-HSPB7 (zebrafish) C117S

   Plasmid

   #110072

   Purpose

   Express C117S mutant of Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Cysteine 117 to Serine

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His10-Draper delta ITAM

   Plasmid

   #126671

   Purpose

   Expression of His10-Draper delta ITAM mutant for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper cytoplasmic ITAM residues mutated to Phe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cytoplasmic ITAM residues mutated to Phe

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198467

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-6xHis-MBP-TEV site-TadA8r

    Plasmid

    #208318

    Purpose

    Overxpression MBP-TadA8r

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET His6 LIC cloning vector (2Bc-T)

    Plasmid

    #37236

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid

    #73440

    Purpose

    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC-P3A2-vioD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p36-p37-p38-p40-pET-Duet-1

    Plasmid

    #175049

    Purpose

    Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169192

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Unspecified

    Tags

    6His-TEV

    Expression

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 TEV LIC cloning vector (2B-T)

    Plasmid

    #29666

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET His6 TEV LIC cloning vector (1B)

    Plasmid

    #29653

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge