19,635 results

1. CD47-bio-His

   Plasmid

   #53150

   Purpose

   Expresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD47 (CD47 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-SNAP-PI(3,5)P2 Probe

   Plasmid

   #211512

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   SnxA-2xPX
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNeonGreen-peroxi

   Plasmid

   #137806

   Purpose

   Visualization of peroxisomes

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   SKL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-luciferase

   Plasmid

   #55764

   Purpose

   luciferase reporter for in vivo bioluminescence imaging

   Depositor

   Snorri Thorgeirsson

   Article

   Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

   Insert

   CMV enhancer/chicken beta actin promoter
   

   Expression

   Mammalian

   Promoter

   CMV enhancer/chicken beta actin promoter

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1-HyPerRed-mito

   Plasmid

   #60247

   Purpose

   Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HyPerRed-mito
   

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET empty polycistronic destination vector (2D)

   Plasmid

   #29774

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS_sfGPF 150TAG

   Plasmid

   #154765

   Purpose

   plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-GST-His6-2_HBD

   Plasmid

   #171870

   Purpose

   Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

   Depositor

   Kaiwei Liang

   Article

   Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

   Insert

   Tandem repeats Hybrid binding domain of Rnase H1
   

   Tags

   GST- and His10-tag

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pROSA26-PA

   Plasmid

   #21271

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Available Since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBbi_RP_MicroID2-HA

    Plasmid

    #188521

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mOrange2-C1

    Plasmid

    #54650

    Purpose

    Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Roger Tsien, Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    mOrange2

    Expression

    Mammalian

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Q23

    Plasmid

    #40261

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. shYAP1 # 1

    Plasmid

    #42540

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-EF1a/b-actin

    Plasmid

    #82583

    Purpose

    5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

    Plasmid

    #160369

    Purpose

    Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    GPR143 (GPR143 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-3xHA

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIL-eGFP

    Plasmid

    #52675

    Purpose

    shRNA silencing plasmid with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Type

    Empty backbone

    Use

    RNAi

    Tags

    CMV-EGFP

    Promoter

    CMV and U6

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_hSyn1-SIO-stGtACR2-FusionRed

    Plasmid

    #105677

    Purpose

    Expresses a soma-targeted GtACR2 in Cre-expressing neurons

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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