Purpose
Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
Insert
gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
Available Since
Jan. 29, 2024
Availability
Academic Institutions and Nonprofits only