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pCDH-EFS-FlpO

Plasmid

#191509

Purpose

Lentiviral Flp Recombinase driven by the EFS promoter

Depositor

Eric Snyder

Article

Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

Insert

flp recombinase (flp )

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a/EFS promoter inserted

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

Plasmid

#128404

Purpose

Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A (toxA P. aeruginosa)

Tags

6xHis tag and DsbA signal sequence for periplasmi…

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A DHFR.cHSF1

Plasmid

#134737

Purpose

Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

DHFR

Mutation

Deletion of amino acids 186-202

Promoter

None

Available Since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-mIL15-IL12rb1-sushi-il12rb2

Plasmid

#174606

Purpose

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ha-Brca1 S1423A/S1524A

Plasmid

#32539

Depositor

Michael Kastan

Article

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Mutation

S1423A/S1524A

Promoter

CMV/rabbit B-Globin

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pTrc99S-ssDsbA-TTlight-4xDQNAT

Plasmid

#128402

Purpose

Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCosSAD1AprR

Plasmid

#190786

Purpose

An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

Depositor

Jan Kormanec

Article

Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

Inserts

upstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-nsp1-c3xFLAG

Plasmid

#215694

Purpose

Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp1

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp2-c3xFLAG

Plasmid

#215695

Purpose

Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp2

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp4-c3xFLAG

Plasmid

#215697

Purpose

Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp4

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

Plasmid

#172226

Purpose

Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

SOX17 (SOX17 Human)

Use

Piggybac transposon

Tags

3XFLAG, DD-domain, GS-linker, and TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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HOXB2 (human) HIS-tag pET

Plasmid

#8522

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB2 (HOXB2 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Starts at AA #2; makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Article

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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UBQLN2_450-624W

Plasmid

#176852

Purpose

Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

Depositor

Carlos Castañeda

Article

Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

Insert

UBQLN2_450C_W (UBQLN2 Human)

Expression

Bacterial

Mutation

deletion of residues 1-449 and addition of C-term…

Promoter

T7

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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DDX5 D248N V5

Plasmid

#199577

Purpose

Expresses helicase dead DDX5 cloned into pLenti puro

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

DDX5 (DDX5 Human)

Tags

V5

Expression

Mammalian

Mutation

D248N

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h

Plasmid

#73066

Purpose

expression clone for human SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133-mut

Plasmid

#87339

Purpose

To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes; deletion of Nup133 AA1-7

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBI101-pGC1(D1)-GUS

Plasmid

#49513

Purpose

Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant

Depositor

Julian Schroeder

Article

Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.

Insert

Promoter of GC1(D1) (AT1G22690 Mustard Weed)

Use

Plant expression

Tags

beta-glucuronidase

Promoter

GC1 (At1g22690), 1141 bp upstream of the transcri…

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-BcRF1-2xStrep

Plasmid

#138436

Purpose

Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Tags

Strep

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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